Cast your vote
You can rate an item by clicking the amount of stars they wish to award to this item.
When enough users have cast their vote on this item, the average rating will also be shown.
Your vote was cast
Thank you for your feedback
Thank you for your feedback
MetadataShow full item record
AbstractDue to their strong antimicrobial activity, silver nanoparticles (AgNPs) are massively produced, applied, consumed and, as a negative consequence, released into wastewater treatment plants. Most AgNPs are assumed to be bound by sludge, and thus bear potential risk for microbial performance and stability. In this lab-scale study, flow cytometry as a high-throughput method and 16S rRNA gene amplicon Illumina MiSeq sequencing were used to track microbial community structure changes when being exposed to AgNPs. Both methods allowed deeper investigation of the toxic impact of chemicals on microbial communities than classical EC50 determination. In addition, ecological metrics were used to quantify microbial community variations depending on AgNP types (10 and 30 nm) and concentrations. Only low changes in α- and intra-community β-diversity values were found both in successive negative and positive control batches and batches that were run with AgNPs below the EC50 value. Instead, AgNPs at EC50 concentrations caused upcoming of certain and disappearance of formerly dominant subcommunities. Flavobacteriia were among those that almost disappeared, while phylotypes affiliated with Gammaproteobacteria (3.6-fold) and Bacilli (8.4-fold) increased in cell abundance in comparison to the negative control. Thus, silver amounts at the EC50 value affected community structure suggesting a potential negative impact on functions in wastewater treatment systems.
AffiliationHZI,Helmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7,38124 Braunschweig, Germany.
JournalFrontiers in Microbiology
The following license files are associated with this item:
- Creative Commons
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-ShareAlike 4.0 International
- Transformation of PVP coated silver nanoparticles in a simulated wastewater treatment process and the effect on microbial communities.
- Authors: Doolette CL, McLaughlin MJ, Kirby JK, Batstone DJ, Harris HH, Ge H, Cornelis G
- Issue date: 2013
- An investigation into the effects of silver nanoparticles on natural microbial communities in two freshwater sediments.
- Authors: Bao S, Wang H, Zhang W, Xie Z, Fang T
- Issue date: 2016 Dec
- Pyrosequencing reveals higher impact of silver nanoparticles than Ag+ on the microbial community structure of activated sludge.
- Authors: Yang Y, Quensen J, Mathieu J, Wang Q, Wang J, Li M, Tiedje JM, Alvarez PJ
- Issue date: 2014 Jan 1
- Bacterial response to a continuous long-term exposure of silver nanoparticles at sub-ppm silver concentrations in a membrane bioreactor activated sludge system.
- Authors: Zhang C, Liang Z, Hu Z
- Issue date: 2014 Mar 1
- Influence of Silver nanoparticles on nutrient removal and microbial communities in SBR process after long-term exposure.
- Authors: Zhang Z, Gao P, Li M, Cheng J, Liu W, Feng Y
- Issue date: 2016 Nov 1