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dc.contributor.authorGrobe, Svenja
dc.contributor.authorDoberenz, Sebastian
dc.contributor.authorFerreira, Kevin
dc.contributor.authorKrueger, Jonas
dc.contributor.authorBrönstrup, Mark
dc.contributor.authorKaever, Volkhard
dc.contributor.authorHäußler, Susanne
dc.date.accessioned2019-02-12T09:29:19Z
dc.date.available2019-02-12T09:29:19Z
dc.date.issued2019-01-15
dc.identifier.citationChembiochem. 2019 Jan 15. doi: 10.1002/cbic.201800741en_US
dc.identifier.issn1439-7633
dc.identifier.pmid30644616
dc.identifier.doi10.1002/cbic.201800741
dc.identifier.urihttp://hdl.handle.net/10033/621684
dc.description.abstractTransfer RNA (tRNA) modifications impact the structure and function of tRNAs thus affecting the efficiency and fidelity of translation. In the opportunistic pathogen Pseudomonas aeruginosa translational regulation plays an important but less defined role in the adaptation to changing environments. In this study, we explored tRNA modifications in P. aeruginosa using LC-MS/MS based approaches. Neutral Loss Scan (NLS) demonstrated the potential to identify previously unknown modifications, while Multiple Reaction Monitoring (MRM) can detect modifications with high specificity and sensitivity. In this study, the MRM-based external calibration method allowed for quantification of the 4 canonical and 32 modified ribonucleosides, of which 21 tRNA modifications were quantified in the total tRNA pool of P. aeruginosa PA14. We also purified the single tRNA isoacceptors tRNA-ArgUCU, tRNA-LeuCAA and tRNA-TrpCCA and determined, both qualitatively and quantitatively, their specific modification pattern. Deeper insights into the nature and dynamics of tRNA modifications in P. aeruginosa will pave the way for further studies on posttranscriptional gene regulation as a relatively unexplored molecular mechanism of controlling bacterial pathogenicity and life style.en_US
dc.language.isoenen_US
dc.publisherWiley-Blackwellen_US
dc.rightsAttribution-NonCommercial-ShareAlike 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.subjectMass spectrometryen_US
dc.subjectPseudomonas aeruginosaen_US
dc.subjecttRNAen_US
dc.subjecttRNA modificationsen_US
dc.titleIdentification and quantification of (t)RNA modifications in Pseudomonas aeruginosa by liquid chromatography-tandem mass spectrometry.en_US
dc.typeArticleen_US
dc.contributor.departmentHZI,Helmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7,38124 Braunschweig, Germany.en_US
dc.source.journaltitleChembiochem : a European journal of chemical biology


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