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dc.contributor.authorRatuszny, Dominica
dc.contributor.authorSühs, Kurt-Wolfram
dc.contributor.authorNovoselova, Natalia
dc.contributor.authorKuhn, Maike
dc.contributor.authorKaever, Volkhard
dc.contributor.authorSkripuletz, Thomas
dc.contributor.authorPessler, Frank
dc.contributor.authorStangel, Martin
dc.date.accessioned2019-02-15T13:11:44Z
dc.date.available2019-02-15T13:11:44Z
dc.date.issued2019-01-15
dc.identifier.issn1422-0067
dc.identifier.pmid30650575
dc.identifier.doi10.3390/ijms20020337
dc.identifier.urihttp://hdl.handle.net/10033/621691
dc.description.abstractEnteroviruses are among the most common causes of viral meningitis. Enteroviral meningitis continues to represent diagnostic challenges, as cerebrospinal fluid (CSF) cell numbers (a well validated diagnostic screening tool) may be normal in up to 15% of patients. We aimed to identify potential CSF biomarkers for enteroviral meningitis, particularly for cases with normal CSF cell count. Using targeted liquid chromatography-mass spectrometry, we determined metabolite profiles from patients with enteroviral meningitis (n = 10), and subdivided them into those with elevated (n = 5) and normal (n = 5) CSF leukocyte counts. Non-inflamed CSF samples from patients with Bell’s palsy and normal pressure hydrocephalus (n = 19) were used as controls. Analysis of 91 metabolites revealed considerable metabolic reprogramming in the meningitis samples. It identified phosphatidylcholine PC.ae.C36.3, asparagine, and glycine as an accurate (AUC, 0.92) combined classifier for enterovirus meningitis overall, and kynurenine as a perfect biomarker for enteroviral meningitis with an increased CSF cell count (AUC, 1.0). Remarkably, PC.ae.C36.3 alone emerged as a single accurate (AUC, 0.87) biomarker for enteroviral meningitis with normal cell count, and a combined classifier comprising PC.ae.C36.3, PC.ae.C36.5, and PC.ae.C38.5 achieved nearly perfect classification (AUC, 0.99). Taken together, this analysis reveals the potential of CSF metabolites as additional diagnostic tools for enteroviral meningitis, and likely other central nervous system (CNS) infections.en_US
dc.language.isoenen_US
dc.publisherMPDIen_US
dc.rightsAttribution-NonCommercial-ShareAlike 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.subjectCNS infectionen_US
dc.subjectbiomarkeren_US
dc.subjectcerebrospinal fluiden_US
dc.subjectenterovirusen_US
dc.subjectmeningitisen_US
dc.subjectmetabolomicsen_US
dc.subjectphosphatidylcholinesen_US
dc.titleIdentification of Cerebrospinal Fluid Metabolites as Biomarkers for Enterovirus Meningitis.en_US
dc.typeArticleen_US
dc.contributor.departmentTWINCORE, Zentrum für experimentelle und klinische Infektionsforschung GmbH,Feodor-Lynen Str. 7, 30625 Hannover, Germany.en_US
dc.identifier.journalInternational journal of molecular sciencesen_US
refterms.dateFOA2019-02-15T13:11:45Z
dc.source.journaltitleInternational journal of molecular sciences


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