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dc.contributor.authorHauf, Samuel
dc.contributor.authorHerrmann, Jennifer
dc.contributor.authorMiethke, Marcus
dc.contributor.authorGibhardt, Johannes
dc.contributor.authorCommichau, Fabian M
dc.contributor.authorMüller, Rolf
dc.contributor.authorFuchs, Stephan
dc.contributor.authorHalbedel, Sven
dc.date.accessioned2019-02-18T14:04:06Z
dc.date.available2019-02-18T14:04:06Z
dc.date.issued2019-01-15
dc.identifier.issn1365-2958
dc.identifier.pmid30648305
dc.identifier.doi10.1111/mmi.14205
dc.identifier.urihttp://hdl.handle.net/10033/621694
dc.description.abstractBacteria can cope with toxic compounds such as antibiotics by inducing genes for their detoxification. A common detoxification strategy is compound excretion by ATP-binding cassette (ABC) transporters, which are synthesized upon compound contact. We previously identified the multi drug resistance ABC transporter LieAB in Listeria monocytogenes, a Gram-positive bacterium that occurs ubiquitously in the environment, but also causes severe infections in humans upon ingestion. Expression of the lieAB genes is strongly induced in cells lacking the PadR-type transcriptional repressor LftR, but compounds leading to relief of this repression in wild type cells were not known. Using RNA-Seq and promoter-lacZ fusions we demonstrate highly specific repression of the lieAB and lftRS promoters through LftR. Screening of a natural compound library yielded the depsipeptide aurantimycin A - synthesized by the soil-dwelling Streptomyces aurantiacus - as the first known naturally occurring inducer of lieAB expression. Genetic and phenotypic experiments concordantly show that aurantimycin A is a substrate of the LieAB transporter and thus, lftRS and lieAB represent the first known genetic module conferring and regulating aurantimycin A resistance. Collectively, these genes may support the survival of L. monocytogenes when it comes into contact with antibiotic-producing bacteria in the soil. This article is protected by copyright. All rights reserved.en_US
dc.language.isoenen_US
dc.publisherWiley-Blackwellen_US
dc.rightsAttribution-NonCommercial-ShareAlike 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.subjectantibiotic resistanceen_US
dc.subjectantimicrobial peptidesen_US
dc.subjectdepsipeptideen_US
dc.subjectdrug effluxen_US
dc.subjectmulti drug resistanceen_US
dc.titleAurantimycin resistance genes contribute to survival of Listeria monocytogenes during life in the environment.en_US
dc.typeArticleen_US
dc.contributor.departmentHIPS, Helmholtz-Institut für Pharmazeutische Forschung Saarland, Universitätscampus E8.1 66123 Saarbrücken, Germany.en_US
dc.identifier.journalMolecular Microbiologyen_US
dc.source.journaltitleMolecular microbiology


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