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dc.contributor.authorCao, Pengbo
dc.contributor.authorWei, Xueming
dc.contributor.authorAwal, Ram Prasad
dc.contributor.authorMüller, Rolf
dc.contributor.authorWall, Daniel
dc.date.accessioned2019-02-25T15:16:42Z
dc.date.available2019-02-25T15:16:42Z
dc.date.issued2019-02-12
dc.identifier.citationMBio. 2019 Feb 12;10(1). pii: mBio.02751-18. doi: 10.1128/mBio.02751-18.en_US
dc.identifier.issn2150-7511
dc.identifier.pmid30755513
dc.identifier.doi10.1128/mBio.02751-18
dc.identifier.urihttp://hdl.handle.net/10033/621705
dc.description.abstractSelf-recognition underlies sociality in many group-living organisms. In bacteria, cells use various strategies to recognize kin to form social groups and, in some cases, to transition into multicellular life. One strategy relies on a single genetic locus that encodes a variable phenotypic tag (“greenbeard”) for recognizing other tag bearers. Previously, we discovered a polymorphic cell surface receptor called TraA that directs self-identification through homotypic interactions in the social bacterium Myxococcus xanthus. Recognition by TraA leads to cellular resource sharing in a process called outer membrane exchange (OME). A second gene in the traA operon, traB, is also required for OME but is not involved in recognition. Our prior studies of TraA identified only six recognition groups among closely related M. xanthus isolates. Here we hypothesize that the number of traA polymorphisms and, consequently, the diversity of recognition in wild isolates are much greater. To test this hypothesis, we expand the scope of TraA characterization to the order Myxococcales. From genomic sequences within the three suborders of Myxococcales, we identified 90 traA orthologs. Sequence analyses and functional characterization of traAB loci suggest that OME is well maintained among diverse myxobacterial taxonomic groups. Importantly, TraA orthologs are highly polymorphic within their variable domain, the region that confers selectivity in self-recognition. We experimentally defined 10 distinct recognition groups and, based on phylogenetic and experimental analyses, predicted >60 recognition groups among the 90 traA alleles. Taken together, our findings revealed a widespread greenbeard locus that mediates the diversity of self-recognition across the order Myxococcales.en_US
dc.language.isoenen_US
dc.publisherAmerican Society of Microbiologyen_US
dc.rightsAttribution-NonCommercial-ShareAlike 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.subjectcell surfaceen_US
dc.subjectcellular transferen_US
dc.subjectkin recognitionen_US
dc.subjectmyxobacteriaen_US
dc.subjectpolymorphismen_US
dc.titleA Highly Polymorphic Receptor Governs Many Distinct Self-Recognition Types within the Myxococcales Order.en_US
dc.typeArticleen_US
dc.contributor.departmentHIPS, Helmholtz-Institut für Pharmazeutische Forschung Saarland, Universitätscampus E8.1 66123 Saarbrücken, Germany.en_US
dc.identifier.journalmBioen_US
refterms.dateFOA2019-02-25T15:16:42Z
atmire.accessrights
dc.source.journaltitlemBio


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