Guidelines for Small-Scale Production and Purification of Hepatitis B Surface Antigen Virus-Like Particles from Recombinant Pichia pastoris.

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Authors
Zahid, Maria
Rinas, Ursula
Issue Date
2019-01-01

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Abstract
Virus-like particle (VLP)-based vaccines have been in the market since decades for preventing viral infection and have proven their usefulness also in other areas of biotechnology. Here, we describe in detail simple small-scale production and purification procedures for the generation of hepatitis B surface antigen (HBsAg) VLPs using Pichia pastoris as expression host. This protocol may also be applicable with variations to other HBsAg-based VLPs additionally carrying antigens of other pathogens.
Citation
Methods Mol Biol. 2019;1923:309-322. doi: 10.1007/978-1-4939-9024-5_14.
Affiliation
HZI,Helmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7,38124 Braunschweig, Germany.
Publisher
Humana Press
Journal
Methods in molecular biology
URI
http://hdl.handle.net/10033/621706
DOI
10.1007/978-1-4939-9024-5_14
PubMed ID
30737747
Type
Article
Language
en
ISSN
1940-6029
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