Guidelines for Small-Scale Production and Purification of Hepatitis B Surface Antigen Virus-Like Particles from Recombinant Pichia pastoris.

Zahid, Maria; Rinas, Ursula

dc.contributor.author	Zahid, Maria
dc.contributor.author	Rinas, Ursula
dc.date.accessioned	2019-02-26T11:35:27Z
dc.date.available	2019-02-26T11:35:27Z
dc.date.issued	2019-01-01
dc.identifier.citation	Methods Mol Biol. 2019;1923:309-322. doi: 10.1007/978-1-4939-9024-5_14.	en_US
dc.identifier.issn	1940-6029
dc.identifier.pmid	30737747
dc.identifier.doi	10.1007/978-1-4939-9024-5_14
dc.identifier.uri	http://hdl.handle.net/10033/621706
dc.description.abstract	Virus-like particle (VLP)-based vaccines have been in the market since decades for preventing viral infection and have proven their usefulness also in other areas of biotechnology. Here, we describe in detail simple small-scale production and purification procedures for the generation of hepatitis B surface antigen (HBsAg) VLPs using Pichia pastoris as expression host. This protocol may also be applicable with variations to other HBsAg-based VLPs additionally carrying antigens of other pathogens.	en_US
dc.language.iso	en	en_US
dc.publisher	Humana Press	en_US
dc.rights	Attribution-NonCommercial-ShareAlike 4.0 International	*
dc.rights.uri	http://creativecommons.org/licenses/by-nc-sa/4.0/	*
dc.subject	HBsAg	en_US
dc.subject	Pichia pastoris	en_US
dc.subject	Vaccine	en_US
dc.subject	Virus	en_US
dc.subject	Virus-like particle	en_US
dc.title	Guidelines for Small-Scale Production and Purification of Hepatitis B Surface Antigen Virus-Like Particles from Recombinant Pichia pastoris.	en_US
dc.type	Article	en_US
dc.contributor.department	HZI,Helmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7,38124 Braunschweig, Germany.	en_US
dc.identifier.journal	Methods in molecular biology	en_US
dc.source.journaltitle	Methods in molecular biology (Clifton, N.J.)