Contribution of the Cpx envelope stress system to metabolism and virulence regulation in Salmonella enterica serovar Typhimurium.
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Authors
Subramaniam, SivaramanMüller, Volker S
Hering, Nina A
Mollenkopf, Hans
Becker, Daniel
Heroven, Ann Kathrin
Dersch, Petra

Pohlmann, Anne
Tedin, Karsten
Porwollik, Steffen
McClelland, Michael
Meyer, Thomas F
Hunke, Sabine
Issue Date
2019-01-01
Metadata
Show full item recordAbstract
The Cpx-envelope stress system regulates the expression of virulence factors in many Gram-negative pathogens. In Salmonella enterica serovar Typhimurium deletion of the sensor kinase CpxA but not of the response regulator CpxR results in the down regulation of the key regulator for invasion, HilA encoded by the Salmonella pathogenicity island 1 (SPI-1). Here, we provide evidence that cpxA deletion interferes with dephosphorylation of CpxR resulting in increased levels of active CpxR and consequently in misregulation of target genes. 14 potential operons were identified to be under direct control of CpxR. These include the virulence determinants ecotin, the omptin PgtE, and the SPI-2 regulator SsrB. The Tat-system and the PocR regulator that together promote anaerobic respiration of tetrathionate on 1,2-propanediol are also under direct CpxR control. Notably, 1,2-propanediol represses hilA expression. Thus, our work demonstrates for the first time the involvement of the Cpx system in a complex network mediating metabolism and virulence function.Citation
PLoS One. 2019 Feb 4;14(2):e0211584. doi: 10.1371/journal.pone.0211584. eCollection 2019.Affiliation
HZI,Helmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7,38124 Braunschweig, Germany.Publisher
PLOSPubMed ID
30716090Type
ArticleLanguage
enISSN
1932-6203ae974a485f413a2113503eed53cd6c53
10.1371/journal.pone.0211584
Scopus Count
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