Contribution of the Cpx envelope stress system to metabolism and virulence regulation in Salmonella enterica serovar Typhimurium.
Cast your vote
You can rate an item by clicking the amount of stars they wish to award to this item.
When enough users have cast their vote on this item, the average rating will also be shown.
Your vote was cast
Thank you for your feedback
Thank you for your feedback
Müller, Volker S
Hering, Nina A
Heroven, Ann Kathrin
Meyer, Thomas F
MetadataShow full item record
AbstractThe Cpx-envelope stress system regulates the expression of virulence factors in many Gram-negative pathogens. In Salmonella enterica serovar Typhimurium deletion of the sensor kinase CpxA but not of the response regulator CpxR results in the down regulation of the key regulator for invasion, HilA encoded by the Salmonella pathogenicity island 1 (SPI-1). Here, we provide evidence that cpxA deletion interferes with dephosphorylation of CpxR resulting in increased levels of active CpxR and consequently in misregulation of target genes. 14 potential operons were identified to be under direct control of CpxR. These include the virulence determinants ecotin, the omptin PgtE, and the SPI-2 regulator SsrB. The Tat-system and the PocR regulator that together promote anaerobic respiration of tetrathionate on 1,2-propanediol are also under direct CpxR control. Notably, 1,2-propanediol represses hilA expression. Thus, our work demonstrates for the first time the involvement of the Cpx system in a complex network mediating metabolism and virulence function.
CitationPLoS One. 2019 Feb 4;14(2):e0211584. doi: 10.1371/journal.pone.0211584. eCollection 2019.
AffiliationHZI,Helmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7,38124 Braunschweig, Germany.
The following license files are associated with this item:
- Creative Commons
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-ShareAlike 4.0 International
- Role of the two-component regulator CpxAR in the virulence of Salmonella enterica serotype Typhimurium.
- Authors: Humphreys S, Rowley G, Stevenson A, Anjum MF, Woodward MJ, Gilbert S, Kormanec J, Roberts M
- Issue date: 2004 Aug
- Activation of hilA expression at low pH requires the signal sensor CpxA, but not the cognate response regulator CpxR, in Salmonella enterica serovar Typhimurium.
- Authors: Nakayama SI, Kushiro A, Asahara T, Tanaka RI, Hu L, Kopecko DJ, Watanabe H
- Issue date: 2003 Oct
- CpxR/CpxA Controls scsABCD Transcription To Counteract Copper and Oxidative Stress in Salmonella enterica Serovar Typhimurium.
- Authors: López C, Checa SK, Soncini FC
- Issue date: 2018 Aug 15
- The two-component system CpxR/A represses the expression of Salmonella virulence genes by affecting the stability of the transcriptional regulator HilD.
- Authors: De la Cruz MA, Pérez-Morales D, Palacios IJ, Fernández-Mora M, Calva E, Bustamante VH
- Issue date: 2015
- The transcriptional regulator SsrB is involved in a molecular switch controlling virulence lifestyles of Salmonella.
- Authors: Pérez-Morales D, Banda MM, Chau NYE, Salgado H, Martínez-Flores I, Ibarra JA, Ilyas B, Coombes BK, Bustamante VH
- Issue date: 2017 Jul