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dc.contributor.authorLagkouvardos, Ilias
dc.contributor.authorLesker, Till R
dc.contributor.authorHitch, Thomas C A
dc.contributor.authorGálvez, Eric J C
dc.contributor.authorSmit, Nathiana
dc.contributor.authorNeuhaus, Klaus
dc.contributor.authorWang, Jun
dc.contributor.authorBaines, John F
dc.contributor.authorAbt, Birte
dc.contributor.authorStecher, Bärbel
dc.contributor.authorOvermann, Jörg
dc.contributor.authorStrowig, Till
dc.contributor.authorClavel, Thomas
dc.date.accessioned2019-03-06T09:22:24Z
dc.date.available2019-03-06T09:22:24Z
dc.date.issued2019-02-19
dc.identifier.citationMicrobiome. 2019 Feb 19;7(1):28. doi: 10.1186/s40168-019-0637-2en_US
dc.identifier.issn2049-2618
dc.identifier.pmid30782206
dc.identifier.doi10.1186/s40168-019-0637-2
dc.identifier.urihttp://hdl.handle.net/10033/621712
dc.description.abstractBacteria within family S24-7 (phylum Bacteroidetes) are dominant in the mouse gut microbiota and detected in the intestine of other animals. Because they had not been cultured until recently and the family classification is still ambiguous, interaction with their host was difficult to study and confusion still exists regarding sequence data annotation. We investigated family S24-7 by combining data from large-scale 16S rRNA gene analysis and from functional and taxonomic studies of metagenomic and cultured species. A total of 685 species was inferred by full-length 16S rRNA gene sequence clustering. While many species could not be assigned ecological habitats (93,045 samples analyzed), the mouse was the most commonly identified host (average of 20% relative abundance and nine co-occurring species). Shotgun metagenomics allowed reconstruction of 59 molecular species, of which 34 were representative of the 16S rRNA gene-derived species clusters. In addition, cultivation efforts allowed isolating five strains representing three species, including two novel taxa. Genome analysis revealed that S24-7 spp. are functionally distinct from neighboring families and versatile with respect to complex carbohydrate degradation. We provide novel data on the diversity, ecology, and description of bacterial family S24-7, for which the name Muribaculaceae is proposed.en_US
dc.language.isoenen_US
dc.publisherBioMedCentralen_US
dc.relation.urlhttps://github.com/tillrobin/iMGMCen
dc.relation.urlhttps://microbiomejournal.biomedcentral.com/articles/10.1186/s40168-019-0637-2#Sec2 (Material and Methods, included in article)en
dc.rightsAttribution-NonCommercial-ShareAlike 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.subjectBacterial diversityen_US
dc.subjectBacteroidetesen_US
dc.subjectCultivationen_US
dc.subjectFamily S24-7en_US
dc.subjectHomeothermaceaeen_US
dc.subjectMetagenomic speciesen_US
dc.subjectMouse gut microbiotaen_US
dc.subjectMuribaculaceaeen_US
dc.titleSequence and cultivation study of Muribaculaceae reveals novel species, host preference, and functional potential of this yet undescribed family.en_US
dc.typeArticleen_US
dc.contributor.departmentHZI, Helmholtz Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7, 38124 Braunschweig Germany.en_US
dc.identifier.journalMicrobiomeen_US
dc.description.linkhttps://microbiomejournal.biomedcentral.com/articles/10.1186/s40168-019-0637-2#Sec2 (Materials and Methods, part of Article)en
refterms.dateFOA2019-03-06T09:22:24Z
dc.source.journaltitleMicrobiome


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