A Listeria monocytogenes ST2 clone lacking chitinase ChiB from an outbreak of non-invasive gastroenteritis.
Cast your vote
You can rate an item by clicking the amount of stars they wish to award to this item.
When enough users have cast their vote on this item, the average rating will also be shown.
Your vote was cast
Thank you for your feedback
Thank you for your feedback
MetadataShow full item record
AbstractAn outbreak with a remarkable Listeria monocytogenes clone causing 163 cases of non-invasive listeriosis occurred in Germany in 2015. Core genome multi locus sequence typing grouped non-invasive outbreak isolates and isolates obtained from related food samples into a single cluster, but clearly separated genetically close isolates obtained from invasive listeriosis cases. A comparative genomic approach identified a premature stop codon in the chiB gene, encoding one of the two L. monocytogenes chitinases, which clustered with disease outcome. Correction of this premature stop codon in one representative gastroenteritis outbreak isolate restored chitinase production, but effects in infection experiments were not found. While the exact role of chitinases in virulence of L. monocytogenes is still not fully understood, our results now clearly show that ChiB-derived activity is not required to establish L. monocytogenes gastroenteritis in humans. This limits a possible role of ChiB in human listeriosis to later steps of the infection.
AffiliationHZI, Helmholtz Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7, 38124 Braunschweig Germany.
JournalEmerging microbes & infections
The following license files are associated with this item:
- Creative Commons
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-ShareAlike 4.0 International
- Backtracking and forward checking of human listeriosis clusters identified a multiclonal outbreak linked to <i>Listeria monocytogenes</i> in meat products of a single producer.
- Authors: Lüth S, Halbedel S, Rosner B, Wilking H, Holzer A, Roedel A, Dieckmann R, Vincze S, Prager R, Flieger A, Al Dahouk S, Kleta S
- Issue date: 2020 Dec
- Outbreak of <i>Listeria monocytogenes</i> in South Africa, 2017-2018: Laboratory Activities and Experiences Associated with Whole-Genome Sequencing Analysis of Isolates.
- Authors: Smith AM, Tau NP, Smouse SL, Allam M, Ismail A, Ramalwa NR, Disenyeng B, Ngomane M, Thomas J
- Issue date: 2019 Jul
- Listeria monocytogenes isolates from ready to eat plant produce are diverse and have virulence potential.
- Authors: Smith A, Hearn J, Taylor C, Wheelhouse N, Kaczmarek M, Moorhouse E, Singleton I
- Issue date: 2019 Jun 16
- Retrospective investigation of listeriosis outbreaks in small ruminants using different analytical approaches for whole genome sequencing-based typing of Listeria monocytogenes.
- Authors: Papić B, Kušar D, Zdovc I, Golob M, Pate M
- Issue date: 2020 Jan
- Multi-virulence-locus sequence typing of 4b Listeria monocytogenes isolates obtained from different sources in India over a 10-year period.
- Authors: Doijad S, Lomonaco S, Poharkar K, Garg S, Knabel S, Barbuddhe S, Jayarao B
- Issue date: 2014 Jul