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dc.contributor.authorSun, Yi-Qian
dc.contributor.authorBusche, Tobias
dc.contributor.authorRückert, Christian
dc.contributor.authorPaulus, Constanze
dc.contributor.authorRebets, Yuriy
dc.contributor.authorNovakova, Renata
dc.contributor.authorKalinowski, Jörn
dc.contributor.authorLuzhetskyy, Andriy N
dc.contributor.authorKormanec, Jan
dc.contributor.authorSekurova, Olga N
dc.contributor.authorZotchev, Sergey B
dc.date.accessioned2019-05-07T08:28:13Z
dc.date.available2019-05-07T08:28:13Z
dc.date.issued2017-06-16
dc.identifier.citationACS Synth Biol. 2017 Jun 16;6(6):1026-1033. doi: 10.1021/acssynbio.6b00353. Epub 2017 Mar 3.en_US
dc.identifier.issn2161-5063
dc.identifier.pmid28221784
dc.identifier.doi10.1021/acssynbio.6b00353
dc.identifier.urihttp://hdl.handle.net/10033/621761
dc.description.abstractGenome mining of actinomycete bacteria aims at the discovery of novel bioactive secondary metabolites that can be developed into drugs. A new repressor-based biosensor to detect activated secondary metabolite biosynthesis gene clusters in Streptomyces was developed. Biosynthetic gene clusters for undecylprodigiosin and coelimycin in the genome of Streptomyces lividans TK24, which encoded TetR-like repressors and appeared to be almost “silent” based on the RNA-seq data, were chosen for the proof-of-principle studies. The bpsA reporter gene for indigoidine synthetase was placed under control of the promotor/operator regions presumed to be controlled by the cluster-associated TetR-like repressors. While the biosensor for undecylprodigiosin turned out to be nonfunctional, the coelimycin biosensor was shown to perform as expected, turning on biosynthesis of indigoidine in response to the concomitant production of coelimycin. The developed reporter system concept can be applied to those cryptic gene clusters that encode metabolite-sensing repressors to speed up discovery of novel bioactive compounds in Streptomyces.en_US
dc.publisherACS Publicationsen_US
dc.relation.urlhttps://app.dimensions.ai/details/publication/pub.1083853402?search_text=10.1021%2Facssynbio.6b00353&search_type=kws&search_field=full_searchen_US
dc.rightsAttribution-NonCommercial-ShareAlike 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.subjectStreptomycesen_US
dc.subjectTetR repressoren_US
dc.subjectbiosensoren_US
dc.subjectorphan gene clustersen_US
dc.subjectsecondary metabolite biosynthesisen_US
dc.titleDevelopment of a Biosensor Concept to Detect the Production of Cluster-Specific Secondary Metabolites.en_US
dc.typeArticleen_US
dc.contributor.departmentHIPS, Helmholtz-Institut für Pharmazeutische Forschung Saarland, Universitätscampus E8.1 66123 Saarbrücken, Germany.en_US
dc.identifier.journalACS Synthetic Biologyen_US
refterms.dateFOA2019-05-07T08:28:14Z
dc.source.journaltitleACS synthetic biology


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