Co-culture of human alveolar epithelial (hAELVi) and macrophage (THP-1) cell lines.
Cast your vote
You can rate an item by clicking the amount of stars they wish to award to this item.
When enough users have cast their vote on this item, the average rating will also be shown.
Your vote was cast
Thank you for your feedback
Thank you for your feedback
de Souza Carvalho-Wodarz, Cristiane
MetadataShow full item record
AbstractThe air-blood barrier is mainly composed of alveolar epithelial cells and macrophages. Whereas the epithelium acts as a diffusional barrier, macrophages represent an immunological barrier, in particular for larger molecules and nanoparticles. This paper describes a new co-culture of human cell lines representing both cell types. Acquiring, culturing and maintaining primary alveolar epithelial cells presents significant logistical and technical difficulties. The recently established human alveolar epithelial lentivirus immortalized cell line, hAELVi, when grown on permeable filters, forms monolayers with high functional and morphological resemblance to alveolar type I cells. To model alveolar macrophages, the human cell line THP-1 was seeded on pre-formed hAELVi monolayers. The co-culture was characterized regarding cellular morphology, viability and barrier function. Macrophages were homogenously distributed on the epithelium and could be kept in co-culture for up to 7 days. Transmission electron microscopy showed loose contact between THP-1 and hAELVi cells. When grown at air liquid interface, both cells were covered with extracellular matrix-like structure, which was absent in THP-1 mono-culture. In co-culture with macrophages, hAELVi cells displayed similar, sometimes even higher, transepithelial electrical resistance than in mono-cultures. When exposed to silver and starch nanoparticles, hAELVi mono-cultures were more tolerant to the particles than THP-1 mono-cultures. Viability in the co-culture was similar to that of hAELVi mono-cultures. Transport studies with sodium fluorescein in the presence/absence of EDTA proved that the co-culture acts as functional diffusion barrier. These data demonstrate that hAELVi-/THP-1 co-cultures represent a promising model for safety and permeability studies of inhaled chemicals, drugs and nanoparticles.
CitationALTEX. 2018;35(2):211-222. doi: 10.14573/altex.1607191. Epub 2017 Nov 23.
AffiliationHIPS, Helmholtz-Institut für Pharmazeutische Forschung Saarland, Universitätscampus E8.1 66123 Saarbrücken, Germany.
The following license files are associated with this item:
- Creative Commons
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-ShareAlike 4.0 International
- Human alveolar epithelial cells expressing tight junctions to model the air-blood barrier.
- Authors: Kuehn A, Kletting S, de Souza Carvalho-Wodarz C, Repnik U, Griffiths G, Fischer U, Meese E, Huwer H, Wirth D, May T, Schneider-Daum N, Lehr CM
- Issue date: 2016
- Triple co-culture of human alveolar epithelium, endothelium and macrophages for studying the interaction of nanocarriers with the air-blood barrier.
- Authors: Costa A, de Souza Carvalho-Wodarz C, Seabra V, Sarmento B, Lehr CM
- Issue date: 2019 Jun
- An in vitro triple cell co-culture model with primary cells mimicking the human alveolar epithelial barrier.
- Authors: Lehmann AD, Daum N, Bur M, Lehr CM, Gehr P, Rothen-Rutishauser BM
- Issue date: 2011 Apr
- Mono- and Cocultures of Bronchial and Alveolar Epithelial Cells Respond Differently to Proinflammatory Stimuli and Their Modulation by Salbutamol and Budesonide.
- Authors: Haghi M, Hittinger M, Zeng Q, Oliver B, Traini D, Young PM, Huwer H, Schneider-Daum N, Lehr CM
- Issue date: 2015 Aug 3
- A responsive human triple-culture model of the air-blood barrier: incorporation of different macrophage phenotypes.
- Authors: Kasper JY, Hermanns MI, Unger RE, Kirkpatrick CJ
- Issue date: 2017 Apr