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dc.contributor.authorAkhwale, Juliah Khayeli
dc.contributor.authorRohde, M
dc.contributor.authorRohde, Christine
dc.contributor.authorBunk, Boyke
dc.contributor.authorSpröer, Cathrin
dc.contributor.authorBoga, Hamadi Iddi
dc.contributor.authorKlenk, Hans-Peter
dc.contributor.authorWittmann, Johannes
dc.date.accessioned2019-05-20T11:45:14Z
dc.date.available2019-05-20T11:45:14Z
dc.date.issued2019-01-01
dc.identifier.citationPLoS One. 2019 Apr 25;14(4):e0215734. doi: 10.1371/journal.pone.0215734. eCollection 2019.en_US
dc.identifier.issn1932-6203
dc.identifier.pmid31022240
dc.identifier.doi10.1371/journal.pone.0215734
dc.identifier.urihttp://hdl.handle.net/10033/621785
dc.description.abstractAs a step towards better understanding of diversity and biology of phages and their hosts in haloalkaline Lake Elmenteita, phages were isolated from sediment samples and overlying water using indigenous bacteria as hosts. 17 seemingly different phages of diverse morphotypes with different dimensions and partly exhibiting remarkably unusual ultrastructures were revealed by transmission electron microscopy. 12 clonal phage isolates were further characterized. Infection capability of the phages was optimum at 30–35°C and in alkali condition with optimum at pH 10–12. Structural protein profiles and restriction fragment length polymorphism analyses patterns were distinct for each of the phage type. Complete nucleotide sequences of phages vB-VmeM-32, vB_EauS-123 and vB_BhaS-171 genomes varied in size from 30,926–199,912 bp and G + C content of between 36.25–47.73%. A range of 56–260 potential open reading frames were identified and annotated. The results showed that the 12 phages were distinct from each other and confirmed the presence and diversity of phages in extreme environment of haloalkaline Lake Elmenteita. The phages were deposited at the German Collection of Microorganisms and Cell Cultures and three of their genomes uploaded to NCBI GenBank.en_US
dc.description.sponsorshipAs a step towards better understanding of diversity and biology of phages and their hosts in haloalkaline Lake Elmenteita, phages were isolated from sediment samples and overlying water using indigenous bacteria as hosts. 17 seemingly different phages of diverse morphotypes with different dimensions and partly exhibiting remarkably unusual ultrastructures were revealed by transmission electron microscopy. 12 clonal phage isolates were further characterized. Infection capability of the phages was optimum at 30-35°C and in alkali condition with optimum at pH 10-12. Structural protein profiles and restriction fragment length polymorphism analyses patterns were distinct for each of the phage type. Complete nucleotide sequences of phages vB-VmeM-32, vB_EauS-123 and vB_BhaS-171 genomes varied in size from 30,926-199,912 bp and G + C content of between 36.25-47.73%. A range of 56-260 potential open reading frames were identified and annotated. The results showed that the 12 phages were distinct from each other and confirmed the presence and diversity of phages in extreme environment of haloalkaline Lake Elmenteita. The phages were deposited at the German Collection of Microorganisms and Cell Cultures and three of their genomes uploaded to NCBI GenBank.en_US
dc.language.isoenen_US
dc.publisherPLOSen_US
dc.rightsAttribution-NonCommercial-ShareAlike 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.titleIsolation, characterization and analysis of bacteriophages from the haloalkaline lake Elmenteita, Kenya.en_US
dc.typeArticleen_US
dc.contributor.departmentHZI, Helmholtz Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7, 38124 Braunschweig, Germany.en_US
dc.identifier.journalPLOS ONEen_US
refterms.dateFOA2019-05-20T11:45:14Z
dc.source.journaltitlePloS one


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