A high-affinity fluorescence probe for copper(II) ions and its application in fluorescence lifetime correlation spectroscopy.
Cast your vote
You can rate an item by clicking the amount of stars they wish to award to this item.
When enough users have cast their vote on this item, the average rating will also be shown.
Your vote was cast
Thank you for your feedback
Thank you for your feedback
MetadataShow full item record
AbstractCopper is one of the most important transition metals in many organisms where it catalyzes a manifold of different processes. As a result of copper's redox activity, organisms have to avoid unbound ions, and a dysfunctional copper homeostasis may lead to multifarious pathological processes in cells with very severe ramifications for the affected organisms. In many neurodegenerative diseases, however, the exact role of copper ions is still not completely clarified. In this work, a high-affinity and highly selective copper probe molecule, based on the naturally occurring tetrapeptide DAHK is synthesized. The sensor (log KD = - 12.8 ± 0.1) is tagged with a fluorescent BODIPY dye whose fluorescence lifetime distinctly decreases from 5.8 ns ± 0.2 ns to 0.4 ns ± 0.1 ns on binding to copper(II) cations. It is shown by using fluorescence lifetime correlation spectroscopy that the concentration of both probe and probe-copper complex can be simultaneously measured even at nanomolar concentration levels. This work presents a possible starting point for a new type of probe and method for future in vivo studies to further reveal the exact role of copper ions in organisms. Graphical abstract.
CitationAnal Bioanal Chem. 2019 Apr 26. pii: 10.1007/s00216-019-01798-y. doi:10.1007/s00216-019-01798-y.
AffiliationHIPS, Helmholtz-Institut für Pharmazeutische Forschung Saarland, Universitätscampus E8.1 66123 Saarbrücken, Germany.
The following license files are associated with this item:
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-ShareAlike 4.0 International
- Development of an oxidative dehydrogenation-based fluorescent probe for Cu2+ and its biological imaging in living cells.
- Authors: Fan J, Liu X, Hu M, Zhu H, Song F, Peng X
- Issue date: 2012 Jul 20
- Highly selective fluorescence turn-on chemosensor based on naphthalimide derivatives for detection of copper(II) ions.
- Authors: Chen Z, Wang L, Zou G, Tang J, Cai X, Teng M, Chen L
- Issue date: 2013 Mar 15
- A sequential and reversibility fluorescent pentapeptide probe for Cu(II) ions and hydrogen sulfide detections and its application in two different living cells imaging.
- Authors: An Y, Wang P, Yue Z
- Issue date: 2019 Jun 5
- Borondipyrromethene-derived Cu2+ sensing chemodosimeter for fast and selective detection.
- Authors: Zhao C, Feng P, Cao J, Wang X, Yang Y, Zhang Y, Zhang J, Zhang Y
- Issue date: 2012 Apr 21
- Fluorescent nanoparticles as selective Cu(II) sensors.
- Authors: Méallet-Renault R, Hérault A, Vachon JJ, Pansu RB, Amigoni-Gerbier S, Larpent C
- Issue date: 2006 Mar