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dc.contributor.authorKoppenhöfer, Sonja
dc.contributor.authorWang, Hui
dc.contributor.authorScharfe, Maren
dc.contributor.authorKaever, Volkhard
dc.contributor.authorWagner-Döbler, Irene
dc.contributor.authorTomasch, Jürgen
dc.date.accessioned2019-06-04T13:38:37Z
dc.date.available2019-06-04T13:38:37Z
dc.date.issued2019-01-01
dc.identifier.citationFront Microbiol. 2019 Apr 12;10:803. doi: 10.3389/fmicb.2019.00803. eCollection 2019.en_US
dc.identifier.issn1664-302X
dc.identifier.pmid31031742
dc.identifier.doi10.3389/fmicb.2019.00803
dc.identifier.urihttp://hdl.handle.net/10033/621798
dc.description.abstractQuorum sensing (QS) coordinates population wide gene expression of bacterial species. Highly adaptive traits like gene transfer agents (GTA), morphological heterogeneity, type 4 secretion systems (T4SS), and flagella are QS controlled in Dinoroseobacter shibae, a Roseobacter model organism. Its QS regulatory network is integrated with the CtrA phosphorelay that controls cell division in alphaproteobacteria. To elucidate the network topology, we analyzed the transcriptional response of the QS-negative D. shibae strain ΔluxI1 toward externally added autoinducer (AI) over a time period of 3 h. The signaling cascade is initiated by the CtrA phosphorelay, followed by the QS genes and other target genes, including the second messenger c-di-GMP, competence, flagella and pili. Identification of transcription factor binding sites in promoters of QS induced genes revealed the integration of QS, CtrA phosphorelay and the SOS stress response mediated by LexA. The concentration of regulatory genes located close to the origin or terminus of replication suggests that gene regulation and replication are tightly coupled. Indeed, addition of AI first stimulates and then represses replication. The restart of replication comes along with increased c-di-GMP levels. We propose a model in which QS induces replication followed by differentiation into GTA producing and non-producing cells. CtrA-activity is controlled by the c-di-GMP level, allowing some of the daughter cells to replicate again. The size of the GTA producing subpopulation is tightly controlled by QS via the AI Synthase LuxI2. Finally, induction of the SOS response allows for integration of GTA DNA into the host chromosome.en_US
dc.language.isoenen_US
dc.publisherFrontiersen_US
dc.rightsAttribution-NonCommercial-ShareAlike 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.subjectCtrAen_US
dc.subjectLexAen_US
dc.subjectRoseobacteren_US
dc.subjectc-di-GMPen_US
dc.subjectchromosome locationen_US
dc.subjectgene transfer agenten_US
dc.subjectquorum sensingen_US
dc.subjectreplicationen_US
dc.titleIntegrated Transcriptional Regulatory Network of Quorum Sensing, Replication Control, and SOS Response in .en_US
dc.typeArticleen_US
dc.contributor.departmentHZI,Helmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7,38124 Braunschweig, Germany.en_US
dc.identifier.journalFrontiers in Microbiologyen_US
refterms.dateFOA2019-06-04T13:38:37Z
dc.source.journaltitleFrontiers in microbiology


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