Essential role of IκB for in vivo CD4 T cell activation, proliferation and Th1 cell differentiation during Listeria monocytogenes infection in mice.
dc.contributor.author | Frentzel, Sarah | |
dc.contributor.author | Katsoulis-Dimitriou, Konstantinos | |
dc.contributor.author | Jeron, Andreas | |
dc.contributor.author | Schmitz, Ingo | |
dc.contributor.author | Bruder, Dunja | |
dc.date.accessioned | 2019-06-05T09:32:03Z | |
dc.date.available | 2019-06-05T09:32:03Z | |
dc.date.issued | 2019-05-03 | |
dc.identifier.citation | Eur J Immunol. 2019 May 3. doi: 10.1002/eji.201847961. | en_US |
dc.identifier.issn | 1521-4141 | |
dc.identifier.pmid | 31049948 | |
dc.identifier.doi | 10.1002/eji.201847961 | |
dc.identifier.uri | http://hdl.handle.net/10033/621800 | |
dc.description.abstract | Acquisition of effector functions in T cells is guided by transcription factors including NF-κB that itself is tightly controlled by inhibitory proteins. The atypical NF-κB inhibitor IκBNS is involved in the development of Th1, Th17 and Treg cells. However, it remained unclear to which extend IκBNS contributes to the acquisition of effector function in T cells specifically responding to a pathogen during in vivo infection. Tracking of adoptively transferred T cells in Listeria monocytogenes infected mice uncovered antigen-specific activation of CD4+ T cells following in vivo pathogen encounter to strongly rely on IκBNS . While IκBNS was largely dispensable for the acquisition of cytotoxic effector function in CD8+ T cells, IκBNS -deficient Th1 effector cells exhibited significantly reduced proliferation, marked changes in the pattern of activation marker expression and reduced production of the Th1-cell cytokines IFNγ, IL2 and TNFα. Complementary in vitro analyses using cells from novel reporter and inducible knockout mice revealed that IκBNS predominantly affects the early phase of Th1-cell differentiation while its function in terminally differentiated cells appears to be negligible. Our data suggest IκBNS as a potential target to modulate specifically CD4+ T-cell responses. This article is protected by copyright. All rights reserved. | en_US |
dc.language.iso | en | en_US |
dc.publisher | Wiley | en_US |
dc.rights | Attribution-NonCommercial-ShareAlike 4.0 International | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-sa/4.0/ | * |
dc.subject | CD4+ T cells | en_US |
dc.subject | IκBNS | en_US |
dc.subject | Listeria monocytogenes | en_US |
dc.subject | Th1 cell differentiation | en_US |
dc.subject | in vivo infection | en_US |
dc.title | Essential role of IκB for in vivo CD4 T cell activation, proliferation and Th1 cell differentiation during Listeria monocytogenes infection in mice. | en_US |
dc.type | Article | en_US |
dc.contributor.department | HZI,Helmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7,38124 Braunschweig, Germany. | en_US |
dc.identifier.journal | European journal of immunology | en_US |
refterms.dateFOA | 2019-06-05T09:32:03Z | |
dc.source.journaltitle | European journal of immunology |