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dc.contributor.authorSeiß, Elena A
dc.contributor.authorKrone, Anna
dc.contributor.authorFormaglio, Pauline
dc.contributor.authorGoldmann, Oliver
dc.contributor.authorEngelmann, Susanne
dc.contributor.authorSchraven, Burkhart
dc.contributor.authorMedina, Eva
dc.contributor.authorMüller, Andreas J
dc.date.accessioned2019-06-07T12:34:07Z
dc.date.available2019-06-07T12:34:07Z
dc.date.issued2019-04-05
dc.identifier.citationSci Rep. 2019 Apr 5;9(1):5703. doi: 10.1038/s41598-019-42129-6.en_US
dc.identifier.issn2045-2322
dc.identifier.pmid30952906
dc.identifier.doi10.1038/s41598-019-42129-6
dc.identifier.urihttp://hdl.handle.net/10033/621806
dc.description.abstractUpon the onset of inflammatory responses, bacterial pathogens are confronted with altered tissue microenvironments which can critically impact on their metabolic activity and growth. Changes in these parameters have however remained difficult to analyze over time, which would be critical to dissect the interplay between the host immune response and pathogen physiology. Here, we established an in vivo biosensor for measuring the growth rates of Staphylococcus aureus (S. aureus) on a single cell-level over days in an ongoing cutaneous infection. Using intravital 2-photon imaging and quantitative fluorescence microscopy, we show that upon neutrophil recruitment to the infection site and bacterial uptake, non-lethal dampening of S. aureus proliferation occurred. This inhibition was supported by NADPH oxidase activity. Therefore, reactive oxygen production contributes to pathogen containment within neutrophils not only by killing S. aureus, but also by restricting the growth rate of the bacterium.en_US
dc.publisherNature publishing groupen_US
dc.rightsAttribution-NonCommercial-ShareAlike 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.titleLongitudinal proliferation mapping in vivo reveals NADPH oxidase-mediated dampening of Staphylococcus aureus growth rates within neutrophils.en_US
dc.typeArticleen_US
dc.contributor.departmentHZI,Helmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7,38124 Braunschweig, Germany.en_US
dc.identifier.journalScientific Reportsen_US
refterms.dateFOA2019-06-07T12:34:08Z
dc.source.journaltitleScientific reports


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Attribution-NonCommercial-ShareAlike 4.0 International
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