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dc.contributor.authorKugelmann, Daniela
dc.contributor.authorRötzer, Vera
dc.contributor.authorWalter, Elias
dc.contributor.authorEgu, Desalegn Tadesse
dc.contributor.authorFuchs, Michael Tobias
dc.contributor.authorVielmuth, Franziska
dc.contributor.authorVargas-Robles, Hilda
dc.contributor.authorSchnoor, Michael
dc.contributor.authorHertl, Michael
dc.contributor.authorEming, Rüdiger
dc.contributor.authorRottner, Klemens
dc.contributor.authorSchmidt, Ansgar
dc.contributor.authorSpindler, Volker
dc.contributor.authorWaschke, Jens
dc.date.accessioned2019-06-12T11:55:19Z
dc.date.available2019-06-12T11:55:19Z
dc.date.issued2019-01-01
dc.identifier.citationFront Immunol. 2019 Apr 4;10:626. doi: 10.3389/fimmu.2019.00626. eCollection 2019.en_US
dc.identifier.issn1664-3224
dc.identifier.pmid31024527
dc.identifier.doi10.3389/fimmu.2019.00626
dc.identifier.urihttp://hdl.handle.net/10033/621815
dc.description.abstractAutoantibodies against desmoglein (Dsg) 1 and Dsg3 primarily cause blister formation in the autoimmune disease pemphigus vulgaris (PV). Src was proposed to contribute to loss of keratinocyte cohesion. However, the role and underlying mechanisms are unclear and were studied here. In keratinocytes, cell cohesion in response to autoantibodies was reduced in Src-dependent manner by two patient-derived PV-IgG fractions as well as by AK23 but not by a third PV-IgG fraction, although Src was activated by all autoantibodies. Loss of cell cohesion was progredient in a timeframe of 24 h and AK23, similar to PV-IgG, interfered with reconstitution of cell cohesion after Ca2+-switch, indicating that the autoantibodies also interfered with desmosome assembly. Dsg3 co-localized along cell contacts and interacted with the Src substrate cortactin. In keratinocytes isolated from cortactin-deficient mice, cell adhesion was impaired and Src-mediated inhibition of AK23-induced loss of cell cohesion for 24 h was significantly reduced compared to wild-type (wt) cells. Similarly, AK23 impaired reconstitution of cell adhesion was Src-dependent only in the presence of cortactin. Likewise, Src inhibition significantly reduced AK23-induced skin blistering in wt but not cortactin-deficient mice. These data suggest that the Src-mediated long-term effects of AK23 on loss of cell cohesion and skin blistering are dependent on cortactin-mediated desmosome assembly. However, in human epidermis PV-IgG-induced skin blistering and ultrastructural alterations of desmosomes were not affected by Src inhibition, indicating that Src may not be critical for skin blistering in intact human skin, at least when high levels of autoantibodies targeting Dsg1 are present.en_US
dc.language.isoenen_US
dc.publisherFrontiersen_US
dc.rightsAttribution-NonCommercial-ShareAlike 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.subjectadhesionen_US
dc.subjectcortactinen_US
dc.subjectpemphigus vulgaris (PV)en_US
dc.subjectskin blistering diseasesen_US
dc.subjectsrcen_US
dc.titleRole of Src and Cortactin in Pemphigus Skin Blistering.en_US
dc.typeArticleen_US
dc.contributor.departmentHZI,Helmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7,38124 Braunschweig, Germany.en_US
dc.identifier.journalFrontiers in Immunologyen_US
refterms.dateFOA2019-06-12T11:55:19Z
dc.source.journaltitleFrontiers in immunology


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