Still Something to Discover: Novel Insights into Phage Diversity and Taxonomy.
Cast your vote
You can rate an item by clicking the amount of stars they wish to award to this item.
When enough users have cast their vote on this item, the average rating will also be shown.
Your vote was cast
Thank you for your feedback
Thank you for your feedback
AuthorsKorf, Imke H E
Meier-Kolthoff, Jan P
Adriaenssens, Evelien M
Kropinski, Andrew M
van Raaij, Mark J
MetadataShow full item record
AbstractThe aim of this study was to gain further insight into the diversity of Escherichia coli phagesfollowed by enhanced work on taxonomic issues in that field. Therefore, we present the genomiccharacterization and taxonomic classification of 50 bacteriophages against E. coli isolated fromvarious sources, such as manure or sewage. All phages were examined for their host range on a setof different E. coli strains, originating, e.g., from human diagnostic laboratories or poultry farms.Transmission electron microscopy revealed a diversity of morphotypes (70% Myo-, 22% Sipho-, and8% Podoviruses), and genome sequencing resulted in genomes sizes from ~44 to ~370 kb.Annotation and comparison with databases showed similarities in particular to T4- and T5-likephages, but also to less-known groups. Though various phages against E. coli are already describedin literature and databases, we still isolated phages that showed no or only few similarities to otherphages, namely phages Goslar, PTXU04, and KWBSE43-6. Genome-based phylogeny andclassification of the newly isolated phages using VICTOR resulted in the proposal of new generaand led to an enhanced taxonomic classification of E. coli phages.
CitationViruses. 2019 May 17;11(5). pii: v11050454. doi: 10.3390/v11050454.
AffiliationHZI,Helmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7,38124 Braunschweig, Germany.
The following license files are associated with this item:
- Creative Commons
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-ShareAlike 4.0 International
- Identification and characterization of new broad host-range rV5-like coliphages C203 and P206 directed against enterobacteria.
- Authors: Sváb D, Falgenhauer L, Rohde M, Chakraborty T, Tóth I
- Issue date: 2018 Oct
- Isolation and characterization of a T4-like phage with a relatively wide host range within Escherichia coli.
- Authors: Xu J, Chen M, He L, Zhang S, Ding T, Yao H, Lu C, Zhang W
- Issue date: 2016 Apr
- Exploring the Remarkable Diversity of Culturable Escherichia coli Phages in the Danish Wastewater Environment.
- Authors: Olsen NS, Forero-Junco L, Kot W, Hansen LH
- Issue date: 2020 Sep 4
- New insights into the biodiversity of coliphages in the intestine of poultry.
- Authors: Sørensen PE, Van Den Broeck W, Kiil K, Jasinskyte D, Moodley A, Garmyn A, Ingmer H, Butaye P
- Issue date: 2020 Sep 16
- Riding the wave of genomics to investigate aquatic coliphage diversity and activity.
- Authors: Michniewski S, Redgwell T, Grigonyte A, Rihtman B, Aguilo-Ferretjans M, Christie-Oleza J, Jameson E, Scanlan DJ, Millard AD
- Issue date: 2019 Jun