Deep sequencing of biofilm microbiomes on dental composite materials.
Cast your vote
You can rate an item by clicking the amount of stars they wish to award to this item.
When enough users have cast their vote on this item, the average rating will also be shown.
Your vote was cast
Thank you for your feedback
Thank you for your feedback
Wendt, Laura Katharina
Abdelbary, Mohamed M H
MetadataShow full item record
AbstractBackground: The microbiome on dental composites has not been studied in detail before. It has not been conclusively clarified whether restorative materials influence the oral microbiome. Methods: We used Illumina Miseq next-generation sequencing of the 16S V1-V2 region to compare the colonisation patterns of bovine enamel (BE) and the composite materials Grandio Flow (GF) and Grandio Blocs (GB) after 48 h in vivo in 14 volunteers. Applying a new method to maintain the oral microbiome ex vivo for 48 h also, we compared the microbiome on GF alone and with the new antimicrobial substance carolacton (GF+C). Results: All in vitro biofilm communities showed a higher diversity and richness than those grown in vivo but the very different atmospheric conditions must be considered. Contrary to expectations, there were only a few significant differences between BE and the composite materials GB and GF either in vivo or in vitro: Oribacterium, Peptostreptococcaceae [XI][G-1] and Streptococcus mutans were more prevalent and Megasphaera, Prevotella oulorum, Veillonella atypica, V. parvula, Gemella morbillorum, and Fusobacterium periodonticum were less prevalent on BE than on composites. In vivo, such preferences were only significant for Granulicatella adiacens (more prevalent on BE) and Fusobacterium nucleatum subsp. animalis (more prevalent on composites). On DNA sequence level, there were no significant differences between the biofilm communities on GF and GF+C. Conclusion: We found that the oral microbiome showed an increased richness when grown on various composites compared to BE in vitro, but otherwise changed only slightly independent of the in vivo or in vitro condition. Our new ex vivo biofilm model might be useful for pre-clinical testing of preventive strategies.
The following license files are associated with this item:
- Creative Commons
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-ShareAlike 4.0 International
- Evaluation of Restorative Materials Containing Preventive Additives in a Secondary Caries Model in vitro.
- Authors: Hetrodt F, Lausch J, Meyer-Lueckel H, Conrads G, Apel C
- Issue date: 2019
- Development and pyrosequencing analysis of an in-vitro oral biofilm model.
- Authors: Kistler JO, Pesaro M, Wade WG
- Issue date: 2015 Feb 10
- Relative presence of Streptococcus mutans, Veillonella atypica, and Granulicatella adiacens in biofilm of complete dentures.
- Authors: Nedumgottil BM
- Issue date: 2018 Jan-Mar
- The Microbiome in Populations with a Low and High Prevalence of Caries.
- Authors: Johansson I, Witkowska E, Kaveh B, Lif Holgerson P, Tanner AC
- Issue date: 2016 Jan
- Dental composite materials containing carolacton inhibit biofilm growth of Streptococcus mutans.
- Authors: Apel C, Barg A, Rheinberg A, Conrads G, Wagner-Döbler I
- Issue date: 2013 Nov