Species-Specific Conservation of Linear Antigenic Sites on Vaccinia Virus A27 Protein Homologs of Orthopoxviruses.
Cast your vote
You can rate an item by clicking the amount of stars they wish to award to this item.
When enough users have cast their vote on this item, the average rating will also be shown.
Your vote was cast
Thank you for your feedback
Thank you for your feedback
AuthorsAhsendorf, Henrike P
Gan, Li L
Eltom, Kamal H
Abd El Wahed, Ahmed
Roper, Rachel L
Hoelzle, Ludwig E
MetadataShow full item record
AbstractThe vaccinia virus (VACV) A27 protein and its homologs, which are found in a large number of members of the genus Orthopoxvirus (OPXV), are targets of viral neutralization by host antibodies. We have mapped six binding sites (epitopes #1A: aa 32-39, #1B: aa 28-33, #1C: aa 26-31, #1D: 28-34, #4: aa 9-14, and #5: aa 68-71) of A27 specific monoclonal antibodies (mAbs) using peptide arrays. MAbs recognizing epitopes #1A-D and #4 neutralized VACV Elstree in a complement dependent way (50% plaque-reduction: 12.5-200 µg/mL). Fusion of VACV at low pH was blocked through inhibition of epitope #1A. To determine the sequence variability of the six antigenic sites, 391 sequences of A27 protein homologs available were compared. Epitopes #4 and #5 were conserved among most of the OPXVs, while the sequential epitope complex #1A-D was more variable and, therefore, responsible for species-specific epitope characteristics. The accurate and reliable mapping of defined epitopes on immuno-protective proteins such as the A27 of VACV enables phylogenetic studies and insights into OPXV evolution as well as to pave the way to the development of safer vaccines and chemical or biological antivirals.
CitationViruses. 2019 May 29;11(6). pii: v11060493. doi: 10.3390/v11060493.
AffiliationHZI,Helmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7,38124 Braunschweig, Germany.
The following license files are associated with this item:
- Creative Commons
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-ShareAlike 4.0 International
- Linear Epitopes in Vaccinia Virus A27 Are Targets of Protective Antibodies Induced by Vaccination against Smallpox.
- Authors: Kaever T, Matho MH, Meng X, Crickard L, Schlossman A, Xiang Y, Crotty S, Peters B, Zajonc DM
- Issue date: 2016 May
- Potent neutralization of vaccinia virus by divergent murine antibodies targeting a common site of vulnerability in L1 protein.
- Authors: Kaever T, Meng X, Matho MH, Schlossman A, Li S, Sela-Culang I, Ofran Y, Buller M, Crump RW, Parker S, Frazier A, Crotty S, Zajonc DM, Peters B, Xiang Y
- Issue date: 2014 Oct
- Epitope detection in the envelope of intracellular naked orthopox viruses and identification of encoding genes.
- Authors: Czerny CP, Johann S, Hölzle L, Meyer H
- Issue date: 1994 May 1
- An epitope conserved in orthopoxvirus A13 envelope protein is the target of neutralizing and protective antibodies.
- Authors: Xu C, Meng X, Yan B, Crotty S, Deng J, Xiang Y
- Issue date: 2011 Sep 15
- Protective effect of surfactant protein d in pulmonary vaccinia virus infection: implication of A27 viral protein.
- Authors: Perino J, Thielens NM, Crouch E, Spehner D, Crance JM, Favier AL
- Issue date: 2013 Mar 21