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dc.contributor.authorKohn, Timo
dc.contributor.authorHeuer, Anja
dc.contributor.authorJogler, Mareike
dc.contributor.authorVollmers, John
dc.contributor.authorBoedeker, Christian
dc.contributor.authorBunk, Boyke
dc.contributor.authorRast, Patrick
dc.contributor.authorBorchert, Daniela
dc.contributor.authorGlöckner, Ines
dc.contributor.authorFreese, Heike M
dc.contributor.authorKlenk, Hans-Peter
dc.contributor.authorOvermann, Jörg
dc.contributor.authorKaster, Anne-Kristin
dc.contributor.authorRohde, Manfred
dc.contributor.authorWiegand, Sandra
dc.contributor.authorJogler, Christian
dc.identifier.citationFront Microbiol. 2016 Dec 22;7:2079. doi: 10.3389/fmicb.2016.02079. eCollection 2016.en_US
dc.description.abstractMembers of the phylum Planctomycetes are ubiquitous bacteria that dwell in aquatic and terrestrial habitats. While planctomycetal species are important players in the global carbon and nitrogen cycle, this phylum is still undersampled and only few genome sequences are available. Here we describe strain NH11T, a novel planctomycete obtained from a crustacean shell (Wadden Sea, Germany). The phylogenetically closest related cultivated species is Gimesia maris, sharing only 87% 16S rRNA sequence identity. Previous isolation attempts have mostly yielded members of the genus Rhodopirellula from water of the German North Sea. On the other hand, only one axenic culture of the genus Pirellula was obtained from a crustacean thus far. However, the 16S rRNA gene sequence of strain NH11T shares only 80% sequence identity with the closest relative of both genera, Rhodopirellula and Pirellula. Thus, strain NH11T is unique in terms of origin and phylogeny. While the pear to ovoid shaped cells of strain NH11T are typical planctomycetal, light-, and electron microscopic observations point toward an unusual variation of cell division through budding: during the division process daughter- and mother cells are connected by an unseen thin tubular-like structure. Furthermore, the periplasmic space of strain NH11T was unusually enlarged and differed from previously known planctomycetes. The complete genome of strain NH11T, with almost 9 Mb in size, is among the largest planctomycetal genomes sequenced thus far, but harbors only 6645 protein-coding genes. The acquisition of genomic components by horizontal gene transfer is indicated by the presence of numerous putative genomic islands. Strikingly, 45 "giant genes" were found within the genome of NH11T. Subsequent analysis of all available planctomycetal genomes revealed that Planctomycetes as such are especially rich in "giant genes". Furthermore, Multilocus Sequence Analysis (MLSA) tree reconstruction support the phylogenetic distance of strain NH11T from other cultivated Planctomycetes of the same phylogenetic cluster. Thus, based on our findings, we propose to classify strain NH11T as Fuerstia marisgermanicae gen. nov., sp. nov., with the type strain NH11T, within the phylum Planctomycetes.en_US
dc.rightsAttribution-NonCommercial-ShareAlike 4.0 International*
dc.subjectFuerstia marisgermanicaeen_US
dc.subjectanimal associateden_US
dc.subjectcell divisionen_US
dc.subjectgiant genesen_US
dc.titleFuerstia marisgermanicae gen. nov., sp. nov., an Unusual Member of the Phylum Planctomycetes from the German Wadden Sea.en_US
dc.contributor.departmentHZI,Helmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7,38124 Braunschweig, Germany.en_US
dc.identifier.journalFrontiers in Microbiologyen_US
dc.source.journaltitleFrontiers in microbiology

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