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dc.contributor.authorMayer, Janine
dc.contributor.authorPippel, Jan
dc.contributor.authorGünther, Gabriele
dc.contributor.authorMüller, Carolin
dc.contributor.authorLauermann, Anna
dc.contributor.authorKnuuti, Tobias
dc.contributor.authorBlankenfeldt, Wulf
dc.contributor.authorJahn, Dieter
dc.contributor.authorBiedendieck, Rebekka
dc.date.accessioned2019-07-16T12:12:45Z
dc.date.available2019-07-16T12:12:45Z
dc.date.issued2019-06-21
dc.identifier.citationAppl Microbiol Biotechnol. 2019 Jun 21. pii: 10.1007/s00253-019-09977-8. doi: 10.1007/s00253-019-09977-8.en_US
dc.identifier.issn1432-0614
dc.identifier.pmid31227867
dc.identifier.doi10.1007/s00253-019-09977-8
dc.identifier.urihttp://hdl.handle.net/10033/621878
dc.description.abstractPenicillin G acylase (PGA) catalyzes the hydrolysis of penicillin G to 6-aminopenicillanic acid and phenylacetic acid, which provides the precursor for most semisynthetic penicillins. Most applications rely on PGAs from Gram-negative bacteria. Here we describe the first three crystal structures for PGAs from Gram-positive Bacilli and their utilization in protein engineering experiments for the manipulation of their thermostability. PGAs from Bacillus megaterium (BmPGA, Tm = 56.0 °C), Bacillus thermotolerans (BtPGA, Tm = 64.5 °C), and Bacillus sp. FJAT-27231 (FJAT-PGA, Tm = 74.3 °C) were recombinantly produced with B. megaterium, secreted, purified to apparent heterogeneity, and crystallized. Structures with resolutions of 2.20 Å (BmPGA), 2.27 Å (BtPGA), and 1.36 Å (FJAT-PGA) were obtained. They revealed high overall similarity, reflecting the high identity of up to approx. 75%. Notably, the active center displays a deletion of more than ten residues with respect to PGAs from Gram-negatives. This enlarges the substrate binding site and may indicate a different substrate spectrum. Based on the structures, ten single-chain FJAT-PGAs carrying artificial linkers were produced. However, in all cases, complete linker cleavage was observed. While thermostability remained in the wild-type range, the enzymatic activity dropped between 30 and 60%. Furthermore, four hybrid PGAs carrying subunits from two different enzymes were successfully produced. Their thermostabilities mostly lay between the values of the two mother enzymes. For one PGA increased, enzyme activity was observed. Overall, the three novel PGA structures combined with initial protein engineering experiments provide the basis for establishment of new PGA-based biotechnological processes.en_US
dc.publisherSpringeren_US
dc.rightsAttribution-NonCommercial-ShareAlike 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.subjectBacillus megateriumen_US
dc.subjectCrystal structureen_US
dc.subjectGram-positiveen_US
dc.subjectPenicillin G acylaseen_US
dc.subjectThermostableen_US
dc.titleCrystal structures and protein engineering of three different penicillin G acylases from Gram-positive bacteria with different thermostability.en_US
dc.typeArticleen_US
dc.contributor.departmentHZI,Helmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7,38124 Braunschweig, Germany.en_US
dc.identifier.journalApplied Microbiology and Biotechnologyen_US
dc.source.journaltitleApplied microbiology and biotechnology


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