Cast your vote
You can rate an item by clicking the amount of stars they wish to award to this item.
When enough users have cast their vote on this item, the average rating will also be shown.
Your vote was cast
Thank you for your feedback
Thank you for your feedback
MetadataShow full item record
AbstractGenome editing is essential for probing genotype-phenotype relationships and for enhancing chemical production and phenotypic robustness in industrial bacteria. Currently, the most popular tools for genome editing couple recombineering with DNA cleavage by the CRISPR nuclease Cas9 from Streptococcus pyogenes. Although successful in some model strains, CRISPR-based genome editing has been slow to extend to the multitude of industrially relevant bacteria. In this review, we analyze existing barriers to implementing CRISPR-based editing across diverse bacterial species. We first compare the efficacy of current CRISPR-based editing strategies. Next, we discuss alternatives when the S. pyogenes Cas9 does not yield colonies. Finally, we describe different ways bacteria can evade editing and how elucidating these failure modes can improve CRISPR-based genome editing across strains. Together, this review highlights existing obstacles to CRISPR-based editing in bacteria and offers guidelines to help achieve and enhance editing in a wider range of bacterial species, including non-model strains.
CitationJ Ind Microbiol Biotechnol. 2019 Jun 5. pii: 10.1007/s10295-019-02195-1. doi: 10.1007/s10295-019-02195-1.
AffiliationHIRI, Helmholtz-Institut für RNA-basierte Infektionsforschung, Josef-Shneider Strasse 2, 97080 Würzburg, Germany.
The following license files are associated with this item:
- Creative Commons
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-ShareAlike 4.0 International
- Challenges of in vitro genome editing with CRISPR/Cas9 and possible solutions: A review.
- Authors: Ebrahimi V, Hashemi A
- Issue date: 2020 Aug 30
- Development of a CRISPR/Cas9 System for Methylococcus capsulatus In Vivo Gene Editing.
- Authors: Tapscott T, Guarnieri MT, Henard CA
- Issue date: 2019 Jun 1
- Genome Editing with CRISPR-Cas9 in Lactobacillus plantarum Revealed That Editing Outcomes Can Vary Across Strains and Between Methods.
- Authors: Leenay RT, Vento JM, Shah M, Martino ME, Leulier F, Beisel CL
- Issue date: 2019 Mar
- Extending CRISPR-Cas9 Technology from Genome Editing to Transcriptional Engineering in the Genus Clostridium.
- Authors: Bruder MR, Pyne ME, Moo-Young M, Chung DA, Chou CP
- Issue date: 2016 Oct 15
- Efficient and Scalable Precision Genome Editing in Staphylococcus aureus through Conditional Recombineering and CRISPR/Cas9-Mediated Counterselection.
- Authors: Penewit K, Holmes EA, McLean K, Ren M, Waalkes A, Salipante SJ
- Issue date: 2018 Feb 20