Bioinspired Liposomes for Oral Delivery of Colistin to Combat Intracellular Infections by Salmonella enterica.
Cast your vote
You can rate an item by clicking the amount of stars they wish to award to this item.
When enough users have cast their vote on this item, the average rating will also be shown.
Your vote was cast
Thank you for your feedback
Thank you for your feedback
Kamal, Mohamed Ashraf M
MetadataShow full item record
AbstractBacterial invasion into eukaryotic cells and the establishment of intracellular infection has proven to be an effective means of resisting antibiotic action, as anti-infective agents commonly exhibit a poor permeability across the host cell membrane. Encapsulation of anti-infectives into nanoscaled delivery systems, such as liposomes, is shown to result in an enhancement of intracellular delivery. The aim of the current work is, therefore, to formulate colistin, a poorly permeable anti-infective, into liposomes suitable for oral delivery, and to functionalize these carriers with a bacteria-derived invasive moiety to enhance their intracellular delivery. Different combinations of phospholipids and cholesterol are explored to optimize liposomal drug encapsulation and stability in biorelevant media. These liposomes are then surface-functionalized with extracellular adherence protein (Eap), derived from Staphylococcus aureus. Treatment of HEp-2 and Caco-2 cells infected with Salmonella enterica using colistin-containing, Eap-functionalized liposomes resulted in a significant reduction of intracellular bacteria, in comparison to treatment with nonfunctionalized liposomes as well as colistin alone. This indicates that such bio-invasive carriers are able to facilitate intracellular delivery of colistin, as necessary for intracellular anti-infective activity. The developed Eap-functionalized liposomes, therefore, present a promising strategy for improving the therapy of intracellular infections.
AffiliationHIPS, Helmholtz-Institut für Pharmazeutische Forschung Saarland, Universitätscampus E8.1 66123 Saarbrücken, Germany.
JournalAdvanced Healthcare Materials
The following license files are associated with this item:
- Creative Commons
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-ShareAlike 4.0 International
- Bacteriomimetic invasin-functionalized nanocarriers for intracellular delivery.
- Authors: Labouta HI, Menina S, Kochut A, Gordon S, Geyer R, Dersch P, Lehr CM
- Issue date: 2015 Dec 28
- Aspherical and Spherical InvA497-Functionalized Nanocarriers for Intracellular Delivery of Anti-Infective Agents.
- Authors: Castoldi A, Empting M, De Rossi C, Mayr K, Dersch P, Hartmann R, Müller R, Gordon S, Lehr CM
- Issue date: 2018 Dec 5
- Antibacterial efficacy of gentamicin encapsulated in pH-sensitive liposomes against an in vivo Salmonella enterica serovar typhimurium intracellular infection model.
- Authors: Cordeiro C, Wiseman DJ, Lutwyche P, Uh M, Evans JC, Finlay BB, Webb MS
- Issue date: 2000 Mar
- Co-Delivery of Ciprofloxacin and Colistin in Liposomal Formulations with Enhanced In Vitro Antimicrobial Activities against Multidrug Resistant Pseudomonas aeruginosa.
- Authors: Wang S, Yu S, Lin Y, Zou P, Chai G, Yu HH, Wickremasinghe H, Shetty N, Ling J, Li J, Zhou QT
- Issue date: 2018 Aug 9
- Intracellular delivery and antibacterial activity of gentamicin encapsulated in pH-sensitive liposomes.
- Authors: Lutwyche P, Cordeiro C, Wiseman DJ, St-Louis M, Uh M, Hope MJ, Webb MS, Finlay BB
- Issue date: 1998 Oct