What you see is what you get: Activity-based probes in single-cell analysis of enzymatic activities
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Authors
Lentz, Christian S.Issue Date
2019-01-01
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Show full item recordAbstract
Molecular imaging methods can provide spatio-temporal information about the distribution of biomolecules or biological processes, such as certain enzymatic activities, in single cells. Within a cell, it is possible to define the subcellular location of a target, its trafficking through the cell, colocalization with other biomolecules of interest and involvement in certain cell biological processes. On the other hand, single-cell imaging promises to distinguish cells that are phenotypically different from each other. The corresponding cellular diversity comprises the presence of functionally distinct cells in a population (‘phenotypic heterogeneity’), as well as dynamic cellular responses to external stimuli (‘phenotypic plasticity’), which is highly relevant, e.g. during cell differentiation, activation (of immune cells), or cell death. This review focuses on applications of a certain class of chemical probes, the so-called activity-based probes (ABPs), for visualization of enzymatic activities in the single-cell context. It discusses the structure of ABPs and other chemical probes, exemplary applications of ABPs in single-cell studies in human, mouse and bacterial systems and considerations to be made with regard to data interpretationAffiliation
HZI,Helmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7,38124 Braunschweig, Germany.Publisher
DeGruyterJournal
Chemical BiologyURI
https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85069763141&origin=inwardhttp://hdl.handle.net/10033/621910
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ArticleLanguage
enISSN
14316730ae974a485f413a2113503eed53cd6c53
10.1515/hsz-2019-0262
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