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dc.contributor.authorLentz, Christian S.
dc.creatorLentz, C.
dc.date.accessioned2019-08-20T11:47:03Z
dc.date.available2019-08-20T11:47:03Z
dc.date.issued2019-01-01
dc.identifier.issn14316730
dc.identifier.doi10.1515/hsz-2019-0262
dc.identifier.urihttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85069763141&origin=inward
dc.identifier.urihttp://hdl.handle.net/10033/621910
dc.description.abstractMolecular imaging methods can provide spatio-temporal information about the distribution of biomolecules or biological processes, such as certain enzymatic activities, in single cells. Within a cell, it is possible to define the subcellular location of a target, its trafficking through the cell, colocalization with other biomolecules of interest and involvement in certain cell biological processes. On the other hand, single-cell imaging promises to distinguish cells that are phenotypically different from each other. The corresponding cellular diversity comprises the presence of functionally distinct cells in a population (‘phenotypic heterogeneity’), as well as dynamic cellular responses to external stimuli (‘phenotypic plasticity’), which is highly relevant, e.g. during cell differentiation, activation (of immune cells), or cell death. This review focuses on applications of a certain class of chemical probes, the so-called activity-based probes (ABPs), for visualization of enzymatic activities in the single-cell context. It discusses the structure of ABPs and other chemical probes, exemplary applications of ABPs in single-cell studies in human, mouse and bacterial systems and considerations to be made with regard to data interpretationen_US
dc.language.isoenen_US
dc.publisherDeGruyteren_US
dc.relation.ispartofBiological Chemistry
dc.rightsAttribution-NonCommercial-ShareAlike 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.subjectactivity-based protein profilingen_US
dc.subjectchemical probeen_US
dc.subjectenzymeen_US
dc.subjectflow cytometryen_US
dc.subjectmolecular imagingen_US
dc.subjectphenotypic heterogeneityen_US
dc.titleWhat you see is what you get: Activity-based probes in single-cell analysis of enzymatic activitiesen_US
dc.typeArticleen_US
dc.contributor.departmentHZI,Helmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7,38124 Braunschweig, Germany.en_US
dc.identifier.journalChemical Biologyen_US
dc.identifier.eid2-s2.0-85069763141
dc.identifier.scopusidSCOPUS_ID:85069763141


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Attribution-NonCommercial-ShareAlike 4.0 International
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-ShareAlike 4.0 International