Molecular profiling of tissue biopsies reveals unique signatures associated with streptococcal necrotizing soft tissue infections
Cast your vote
You can rate an item by clicking the amount of stars they wish to award to this item.
When enough users have cast their vote on this item, the average rating will also be shown.
Your vote was cast
Thank you for your feedback
Thank you for your feedback
Madsen, Martin Bruun
Pieper, Dietmar H.
MetadataShow full item record
AbstractNecrotizing soft tissue infections (NSTIs) are devastating infections caused by either a single pathogen, predominantly Streptococcus pyogenes, or by multiple bacterial species. A better understanding of the pathogenic mechanisms underlying these different NSTI types could facilitate faster diagnostic and more effective therapeutic strategies. Here, we integrate microbial community profiling with host and pathogen(s) transcriptional analysis in patient biopsies to dissect the pathophysiology of streptococcal and polymicrobial NSTIs. We observe that the pathogenicity of polymicrobial communities is mediated by synergistic interactions between community members, fueling a cycle of bacterial colonization and inflammatory tissue destruction. In S. pyogenes NSTIs, expression of specialized virulence factors underlies infection pathophysiology. Furthermore, we identify a strong interferon-related response specific to S. pyogenes NSTIs that could be exploited as a potential diagnostic biomarker. Our study provides insights into the pathophysiology of mono- and polymicrobial NSTIs and highlights the potential of host-derived signatures for microbial diagnosis of NSTIs.
CitationNat Commun. 2019 Aug 26;10(1):3846. doi: 10.1038/s41467-019-11722-8.
AffiliationHZI,Helmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7,38124 Braunschweig, Germany.
The following license files are associated with this item:
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-ShareAlike 4.0 International
- Genetic Architecture of Group A Streptococcal Necrotizing Soft Tissue Infections in the Mouse.
- Authors: Chella Krishnan K, Mukundan S, Alagarsamy J, Hur J, Nookala S, Siemens N, Svensson M, Hyldegaard O, Norrby-Teglund A, Kotb M
- Issue date: 2016 Jul
- Determinants of mortality for necrotizing soft-tissue infections.
- Authors: McHenry CR, Piotrowski JJ, Petrinic D, Malangoni MA
- Issue date: 1995 May
- Pivotal Role of Preexisting Pathogen-Specific Antibodies in the Development of Necrotizing Soft-Tissue Infections.
- Authors: Babbar A, Bruun T, Hyldegaard O, Nekludov M, Arnell P, INFECT Study Group., Pieper DH, Itzek A
- Issue date: 2018 Jun 5
- Comparing culture and molecular methods for the identification of microorganisms involved in necrotizing soft tissue infections.
- Authors: Rudkjøbing VB, Thomsen TR, Xu Y, Melton-Kreft R, Ahmed A, Eickhardt S, Bjarnsholt T, Poulsen SS, Nielsen PH, Earl JP, Ehrlich GD, Moser C
- Issue date: 2016 Nov 8
- Biofilm in group A streptococcal necrotizing soft tissue infections.
- Authors: Siemens N, Chakrakodi B, Shambat SM, Morgan M, Bergsten H, Hyldegaard O, Skrede S, Arnell P, Madsen MB, Johansson L, INFECT Study Group., Juarez J, Bosnjak L, Mörgelin M, Svensson M, Norrby-Teglund A
- Issue date: 2016 Jul 7