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dc.contributor.authorGemperlein, Katja
dc.contributor.authorDietrich, Demian
dc.contributor.authorKohlstedt, Michael
dc.contributor.authorZipf, Gregor
dc.contributor.authorBernauer, Hubert S
dc.contributor.authorWittmann, Christoph
dc.contributor.authorWenzel, Silke C
dc.contributor.authorMüller, Rolf
dc.date.accessioned2019-09-16T09:27:23Z
dc.date.available2019-09-16T09:27:23Z
dc.date.issued2019-09-06
dc.identifier.citationNat Commun. 2019 Sep 6;10(1):4055. doi: 10.1038/s41467-019-12025-8.en_US
dc.identifier.issn2041-1723
dc.identifier.pmid31492836
dc.identifier.doi10.1038/s41467-019-12025-8
dc.identifier.urihttp://hdl.handle.net/10033/621938
dc.description.abstractLong-chain polyunsaturated fatty acids (LC-PUFAs), particularly the omega-3 LC-PUFAs eicosapentaenoic acid (EPA), docosapentaenoic acid (DPA), and docosahexaenoic acid (DHA), have been associated with beneficial health effects. Consequently, sustainable sources have to be developed to meet the increasing demand for these PUFAs. Here, we demonstrate the design and construction of artificial PUFA biosynthetic gene clusters (BGCs) encoding polyketide synthase-like PUFA synthases from myxobacteria adapted for the oleaginous yeast Yarrowia lipolytica. Genomic integration and heterologous expression of unmodified or hybrid PUFA BGCs yielded different yeast strains with specific LC-PUFA production profiles at promising yield and thus valuable for the biotechnological production of distinct PUFAs. Nutrient screening revealed a strong enhancement of PUFA production, when cells were phosphate limited. This represents, to the best of our knowledge, highest concentration of DHA (16.8 %) in total fatty acids among all published PUFA-producing Y. lipolytica strains.en_US
dc.publisherNature publishing Groupen_US
dc.rightsAttribution-NonCommercial-ShareAlike 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.titlePolyunsaturated fatty acid production by Yarrowia lipolytica employing designed myxobacterial PUFA synthases.en_US
dc.typeArticleen_US
dc.contributor.departmentHIPS, Helmholtz-Institut für Pharmazeutische Forschung Saarland, Universitätscampus E8.1 66123 Saarbrücken, Germany.en_US
dc.identifier.journalNature Communicationsen_US
refterms.dateFOA2019-09-16T09:27:24Z
dc.source.journaltitleNature communications


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Except where otherwise noted, this item's license is described as Attribution-NonCommercial-ShareAlike 4.0 International