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dc.contributor.authorPerche, Federico
dc.contributor.authorClemençon, Rudy
dc.contributor.authorSchulze, Kai
dc.contributor.authorEbensen, Thomas
dc.contributor.authorGuzmán, Carlos A
dc.contributor.authorPichon, Chantal
dc.date.accessioned2019-09-16T11:35:19Z
dc.date.available2019-09-16T11:35:19Z
dc.date.issued2019-09-06
dc.identifier.citation- Mol Ther Nucleic Acids. 2019 Sep 6;17:767-775. doi: 10.1016/j.omtn.2019.07.014. Epub 2019 Jul 30.en_US
dc.identifier.issn2162-2531
dc.identifier.pmid31446119
dc.identifier.doi10.1016/j.omtn.2019.07.014
dc.identifier.urihttp://hdl.handle.net/10033/621939
dc.description.abstractNucleic acid vaccination relies on injecting DNA or RNA coding antigen(s) to induce a protective immune response. RNA vaccination is being increasingly used in preclinical and clinical studies. However, few delivery systems have been reported for in vivo delivery of RNA of different sizes. Using a tripartite formulation with RNA, cationic polymer, and anionic liposomes, we were able to encapsulate RNA into neutral lipopolyplexes (LPPs). LPPs were stable in vitro and successfully delivered conventional RNA and replicative RNA to dendritic cells in cellulo. Their injection led to reporter gene expression in mice. Finally, administration of LPP-Replicon RNA (RepRNA) led to an adaptive immune response against the antigen coded by the RepRNA. Accordingly, LPPs may represent a universal formulation for RNA delivery.en_US
dc.language.isoenen_US
dc.publisherElsevier (Cell Press)en_US
dc.rightsAttribution-NonCommercial-ShareAlike 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.subjectmRNA deliveryen_US
dc.subjectself-amplifying RNAen_US
dc.subjectsplenic dendritic cellsen_US
dc.subjecttargetingen_US
dc.titleNeutral Lipopolyplexes for In Vivo Delivery of Conventional and Replicative RNA Vaccine.en_US
dc.typeArticleen_US
dc.contributor.departmentHZI,Helmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7,38124 Braunschweig, Germany.en_US
dc.identifier.journalMolecular Therapy - Nucleic Acidsen_US
refterms.dateFOA2019-09-16T11:35:19Z
dc.source.journaltitleMolecular therapy. Nucleic acids


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Except where otherwise noted, this item's license is described as Attribution-NonCommercial-ShareAlike 4.0 International