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dc.contributor.authorSchaadt, Nadine S
dc.contributor.authorAlfonso, Juan Carlos López
dc.contributor.authorSchönmeyer, Ralf
dc.contributor.authorGrote, Anne
dc.contributor.authorForestier, Germain
dc.contributor.authorWemmert, Cédric
dc.contributor.authorKrönke, Nicole
dc.contributor.authorStoeckelhuber, Mechthild
dc.contributor.authorKreipe, Hans H
dc.contributor.authorHatzikirou, Haralampos
dc.contributor.authorFeuerhake, Friedrich
dc.identifier.citationBreast Cancer Res Treat. 2017 Jul;164(2):305-315. doi: 10.1007/s10549-017-4239-z. Epub 2017 Apr 25.en_US
dc.description.abstractPurpose: To improve microscopic evaluation of immune cells relevant in breast cancer oncoimmunology, we aim at distinguishing normal infiltration patterns from lymphocytic lobulitis by advanced image analysis. We consider potential immune cell variations due to the menstrual cycle and oral contraceptives in non-neoplastic mammary gland tissue. METHODS: Lymphocyte and macrophage distributions were analyzed in the anatomical context of the resting mammary gland in immunohistochemically stained digital whole slide images obtained from 53 reduction mammoplasty specimens. Our image analysis workflow included automated regions of interest detection, immune cell recognition, and co-registration of regions of interest. RESULTS: In normal lobular epithelium, seven CD8[Formula: see text] lymphocytes per 100 epithelial cells were present on average and about 70% of this T-lymphocyte population was lined up along the basal cell layer in close proximity to the epithelium. The density of CD8[Formula: see text] T-cell was 1.6 fold higher in the luteal than in the follicular phase in spontaneous menstrual cycles and 1.4 fold increased under the influence of oral contraceptives, and not co-localized with epithelial proliferation. CD4[Formula: see text] T-cells were infrequent. Abundant CD163[Formula: see text] macrophages were widely spread, including the interstitial compartment, with minor variation during the menstrual cycle. CONCLUSIONS: Spatial patterns of different immune cell subtypes determine the range of normal, as opposed to inflammatory conditions of the breast tissue microenvironment. Advanced image analysis enables quantification of hormonal effects, refines lymphocytic lobulitis, and shows potential for comprehensive biopsy evaluation in oncoimmunologen_US
dc.rightsAttribution-NonCommercial-ShareAlike 4.0 International*
dc.subjectDigital pathologyen_US
dc.subjectHormonal fluctuationsen_US
dc.subjectLymphocytic lobulitisen_US
dc.subjectMenstrual cycleen_US
dc.subjectObject-based image analysisen_US
dc.titleImage analysis of immune cell patterns in the human mammary gland during the menstrual cycle refines lymphocytic lobulitis.en_US
dc.contributor.departmentBRICS, Braunschweiger Zentrum für Systembiologie, Rebenring 56,38106 Braunschweig, Germany.en_US
dc.identifier.journalBrest cancer research and treatmenten_US
dc.source.journaltitleBreast cancer research and treatment

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Attribution-NonCommercial-ShareAlike 4.0 International
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