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dc.contributor.authorMichaux, Charlotte
dc.contributor.authorHolmqvist, Erik
dc.contributor.authorVasicek, Erin
dc.contributor.authorSharan, Malvika
dc.contributor.authorBarquist, Lars
dc.contributor.authorWestermann, Alexander J
dc.contributor.authorGunn, John S
dc.contributor.authorVogel, Jörg
dc.identifier.citationProc Natl Acad Sci U S A. 2017 Jun 27;114(26):6824-6829. doi: 10.1073/pnas.1620772114. Epub 2017 Jun 13.en_US
dc.description.abstractThe functions of many bacterial RNA-binding proteins remain obscure because of a lack of knowledge of their cellular ligands. Although well-studied cold-shock protein A (CspA) family members are induced and function at low temperature, others are highly expressed in infection-relevant conditions. Here, we have profiled transcripts bound in vivo by the CspA family members of Salmonella enterica serovar Typhimurium to link the constitutively expressed CspC and CspE proteins with virulence pathways. Phenotypic assays in vitro demonstrated a crucial role for these proteins in membrane stress, motility, and biofilm formation. Moreover, double deletion of cspC and cspE fully attenuates Salmonella in systemic mouse infection. In other words, the RNA ligand-centric approach taken here overcomes a problematic molecular redundancy of CspC and CspE that likely explains why these proteins have evaded selection in previous virulence factor screens in animals. Our results highlight RNA-binding proteins as regulators of pathogenicity and potential targets of antimicrobial therapy. They also suggest that globally acting RNA-binding proteins are more common in bacteria than currently appreciated.en_US
dc.publisherNational Academy of Sciencesen_US
dc.rightsAttribution-NonCommercial-ShareAlike 4.0 International*
dc.subjectRNA-binding proteinen_US
dc.subjectbacterial pathogenesisen_US
dc.subjectcold-shock proteinen_US
dc.subjectstress responseen_US
dc.titleRNA target profiles direct the discovery of virulence functions for the cold-shock proteins CspC and CspE.en_US
dc.contributor.departmentHIRI, Helmholtz-Institut für RNA-basierte Infektionsforschung, Josef-Shneider Strasse 2, 97080 Würzburg, Germany.en_US
dc.identifier.journalProceedings of the National Academy of Sciences.en_US
dc.source.journaltitleProceedings of the National Academy of Sciences of the United States of America

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