Show simple item record

dc.contributor.authorErdmann, Jelena
dc.contributor.authorThöming, Janne G
dc.contributor.authorPohl, Sarah
dc.contributor.authorPich, Andreas
dc.contributor.authorLenz, Christof
dc.contributor.authorHäussler, Susanne
dc.date.accessioned2019-10-17T12:38:12Z
dc.date.available2019-10-17T12:38:12Z
dc.date.issued2019-09-23
dc.identifier.citationCells. 2019 Sep 23;8(10). pii: cells8101129. doi: 10.3390/cells8101129en_US
dc.identifier.issn2073-4409
dc.identifier.pmid31547513
dc.identifier.doi10.3390/cells8101129
dc.identifier.urihttp://hdl.handle.net/10033/621983
dc.description.abstractComparative genomics has greatly facilitated the identification of shared as well as unique features among individual cells or tissues, and thus offers the potential to find disease markers. While proteomics is recognized for its potential to generate quantitative maps of protein expression, comparative proteomics in bacteria has been largely restricted to the comparison of single cell lines or mutant strains. In this study, we used a data independent acquisition (DIA) technique, which enables global protein quantification of large sample cohorts, to record the proteome profiles of overall 27 whole genome sequenced and transcriptionally profiled clinical isolates of the opportunistic pathogen Pseudomonas aeruginosa. Analysis of the proteome profiles across the 27 clinical isolates grown under planktonic and biofilm growth conditions led to the identification of a core biofilm-associated protein profile. Furthermore, we found that protein-to-mRNA ratios between different P. aeruginosa strains are well correlated, indicating conserved patterns of post-transcriptional regulation. Uncovering core regulatory pathways, which drive biofilm formation and associated antibiotic tolerance in bacterial pathogens, promise to give clues to interactions between bacterial species and their environment and could provide useful targets for new clinical interventions to combat biofilm-associated infections.en_US
dc.publisherMPDIen_US
dc.rightsAttribution-NonCommercial-ShareAlike 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.subjectDIAen_US
dc.subjectSWATHen_US
dc.subjectbacteriaen_US
dc.subjectmass spectrometryen_US
dc.subjectmicrobiologyen_US
dc.titleThe Core Proteome of Biofilm-Grown Clinical Isolates.en_US
dc.typeArticleen_US
dc.contributor.departmentHZI,Helmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7,38124 Braunschweig, Germany.en_US
dc.identifier.journalCellsen_US
refterms.dateFOA2019-10-17T12:38:13Z
dc.source.journaltitleCells


Files in this item

Thumbnail
Name:
Erdmann et al.pdf
Size:
9.454Mb
Format:
PDF
Description:
Open Access publication

This item appears in the following Collection(s)

Show simple item record

Attribution-NonCommercial-ShareAlike 4.0 International
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-ShareAlike 4.0 International