Proteomic and Membrane Lipid Correlates of Reduced Host Defense Peptide.
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Authors
Kohler, ChristianProctor, Richard A
Bayer, Arnold S
Yeaman, Michael R
Lalk, Michael
Engelmann, Susanne
Mishra, Nagendra N
Issue Date
2019-09-28
Metadata
Show full item recordAbstract
We previously described a transposon mutant in Staphylococcus aureus strain SH1000 that exhibited reduced susceptibility to cationic thrombin-induced platelet microbicidal proteins (tPMPs). The transposon insertion site was mapped to the gene snoD, the staphylococcal nuo orthologue. Hence, further studies have been performed to understand how this mutation impacts susceptibility to tPMP, by comparing proteomics profiling and membrane lipid analyses of the parent vs. mutant strains. Surprisingly, the mutant showed differential regulation of only a single protein when cultivated aerobically (FadB), and only a small number of proteins under anaerobic growth conditions (AdhE, DapE, Ddh, Ald1, IlvA1, AgrA, Rot, SA2366, and SA2367). Corresponding to FadB impact on lipid remodeling, membrane fatty acid analyses showed that the snoD mutant contained more short chain anteiso-, but fewer short chain iso-branched chain fatty acids under both aerobic and anaerobic conditions vs. the parental strain. Based upon these proteomic and membrane compositional data, a hypothetical "network" model was developed to explain the impact of the snoD mutation upon tPMP susceptibility.Citation
Antibiotics (Basel). 2019 Sep 28;8(4). pii: antibiotics8040169. doi: 10.3390/antibiotics8040169.Affiliation
HIPS, Helmholtz-Institut für Pharmazeutische Forschung Saarland, Universitätscampus E8.1 66123 Saarbrücken, Germany.Publisher
MPDIJournal
Antibiotics (Basel)PubMed ID
31569354Type
ArticleLanguage
enISSN
2079-6382ae974a485f413a2113503eed53cd6c53
10.3390/antibiotics8040169
Scopus Count
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- Creative Commons
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