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dc.contributor.authorKohler, Christian
dc.contributor.authorProctor, Richard A
dc.contributor.authorBayer, Arnold S
dc.contributor.authorYeaman, Michael R
dc.contributor.authorLalk, Michael
dc.contributor.authorEngelmann, Susanne
dc.contributor.authorMishra, Nagendra N
dc.date.accessioned2019-10-28T14:59:59Z
dc.date.available2019-10-28T14:59:59Z
dc.date.issued2019-09-28
dc.identifier.citationAntibiotics (Basel). 2019 Sep 28;8(4). pii: antibiotics8040169. doi: 10.3390/antibiotics8040169.en_US
dc.identifier.issn2079-6382
dc.identifier.pmid31569354
dc.identifier.doi10.3390/antibiotics8040169
dc.identifier.urihttp://hdl.handle.net/10033/621993
dc.description.abstractWe previously described a transposon mutant in Staphylococcus aureus strain SH1000 that exhibited reduced susceptibility to cationic thrombin-induced platelet microbicidal proteins (tPMPs). The transposon insertion site was mapped to the gene snoD, the staphylococcal nuo orthologue. Hence, further studies have been performed to understand how this mutation impacts susceptibility to tPMP, by comparing proteomics profiling and membrane lipid analyses of the parent vs. mutant strains. Surprisingly, the mutant showed differential regulation of only a single protein when cultivated aerobically (FadB), and only a small number of proteins under anaerobic growth conditions (AdhE, DapE, Ddh, Ald1, IlvA1, AgrA, Rot, SA2366, and SA2367). Corresponding to FadB impact on lipid remodeling, membrane fatty acid analyses showed that the snoD mutant contained more short chain anteiso-, but fewer short chain iso-branched chain fatty acids under both aerobic and anaerobic conditions vs. the parental strain. Based upon these proteomic and membrane compositional data, a hypothetical "network" model was developed to explain the impact of the snoD mutation upon tPMP susceptibility.en_US
dc.language.isoenen_US
dc.publisherMPDIen_US
dc.rightsAttribution-NonCommercial-ShareAlike 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.subjectS. aureusen_US
dc.subjectlipidsen_US
dc.subjectproteomicsen_US
dc.subjectsnoD mutanten_US
dc.subjecttPMP resistanceen_US
dc.titleProteomic and Membrane Lipid Correlates of Reduced Host Defense Peptide.en_US
dc.typeArticleen_US
dc.contributor.departmentHIPS, Helmholtz-Institut für Pharmazeutische Forschung Saarland, Universitätscampus E8.1 66123 Saarbrücken, Germany.en_US
dc.identifier.journalAntibiotics (Basel)en_US
refterms.dateFOA2019-10-28T14:59:59Z
dc.source.journaltitleAntibiotics (Basel, Switzerland)


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