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dc.contributor.authorLevitsky, Lev I
dc.contributor.authorKliuchnikova, Anna A
dc.contributor.authorKuznetsova, Ksenia G
dc.contributor.authorKarpov, Dmitry S
dc.contributor.authorIvanov, Mark V
dc.contributor.authorPyatnitskiy, Mikhail A
dc.contributor.authorKalinina, Olga V
dc.contributor.authorGorshkov, Mikhail V
dc.contributor.authorMoshkovskii, Sergei A
dc.date.accessioned2019-11-05T12:03:59Z
dc.date.available2019-11-05T12:03:59Z
dc.date.issued2019-10-01
dc.identifier.citationProteomics. 2019 Oct 1:e1900195. doi: 10.1002/pmic.201900195.en_US
dc.identifier.issn1615-9861
dc.identifier.pmid31576663
dc.identifier.doi10.1002/pmic.201900195
dc.identifier.urihttp://hdl.handle.net/10033/622001
dc.description.abstractProteogenomics is based on the use of customized genome or RNA sequencing databases for interrogation of shotgun proteomics data in search for proteome-level evidence of genome variations or RNA editing. In this work, we identified the products of adenosine-to-inosine RNA editing in human and murine brain proteomes using publicly available brain proteome LC-MS/MS datasets and an RNA editome database compiled from several sources. After filtering of false-positive results, 20 and 37 sites of editing in proteins belonging to 14 and 32 genes were identified for murine and human brain proteomes, respectively. Eight sites of editing identified with high spectral counts overlapped between human and mouse brain samples. Some of these sites were previously reported using orthogonal methods, such as NMDA glutamate receptors, CYFIP2, coatomer alpha, etc. Also, differential editing between neurons and microglia was demonstrated in this work for some of the proteins from primary murine brain cell cultures. Because many edited sites are still not characterized functionally at the protein level, our results provide a necessary background for their further analysis in normal and diseased cells and tissues using targeted proteomic approaches.en_US
dc.language.isoenen_US
dc.publisherWiley-Blackwellen_US
dc.rightsAttribution-NonCommercial-ShareAlike 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.subjectAMPA glutamate receptoren_US
dc.subjectRNA editingen_US
dc.subjectadenosine deaminaseen_US
dc.subjectbrain proteomeen_US
dc.subjectproteogenomicsen_US
dc.titleAdenosine-to-Inosine RNA Editing in Mouse and Human Brain Proteomes.en_US
dc.typeArticleen_US
dc.contributor.departmentHIPS, Helmholtz-Institut für Pharmazeutische Forschung Saarland, Universitätscampus E8.1 66123 Saarbrücken, Germany.en_US
dc.identifier.journalProteomicsen_US
refterms.dateFOA2019-11-05T12:03:59Z
dc.source.journaltitleProteomics


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