Show simple item record

dc.contributor.authorWibowo, Joko T
dc.contributor.authorKellermann, Matthias Y
dc.contributor.authorVersluis, Dennis
dc.contributor.authorPutra, Masteria Y
dc.contributor.authorMurniasih, Tutik
dc.contributor.authorMohr, Kathrin I
dc.contributor.authorWink, Joachim
dc.contributor.authorEngelmann, Michael
dc.contributor.authorPraditya, Dimas F
dc.contributor.authorSteinmann, Eike
dc.contributor.authorSchupp, Peter J
dc.date.accessioned2019-11-21T09:44:29Z
dc.date.available2019-11-21T09:44:29Z
dc.date.issued2019-11-08
dc.identifier.citationMar Drugs. 2019 Nov 8;17(11). pii: md17110635. doi: 10.3390/md17110635.en_US
dc.identifier.issn1660-3397
dc.identifier.pmid31717405
dc.identifier.doi10.3390/md17110635
dc.identifier.urihttp://hdl.handle.net/10033/622023
dc.description.abstractIn order to minimize re-discovery of already known anti-infective compounds, we focused our screening approach on understudied, almost untapped marine environments including marine invertebrates and their associated bacteria. Therefore, two sea cucumber species, Holothuria leucospilota and Stichopus vastus, were collected from Lampung (Indonesia), and 127 bacterial strains were identified by partial 16S rRNA-gene sequencing analysis and compared with the NCBI database. In addition, the overall bacterial diversity from tissue samples of the sea cucumbers H. leucospilota and S. vastus was analyzed using the cultivation-independent Illumina MiSEQ analysis. Selected bacterial isolates were grown to high densities and the extracted biomass was tested against a selection of bacteria and fungi as well as the hepatitis C virus (HCV). Identification of putative bioactive bacterial-derived compounds were performed by analyzing the accurate mass of the precursor/parent ions (MS1) as well as product/daughter ions (MS2) using high resolution mass spectrometry (HRMS) analysis of all active fractions. With this attempt we were able to identify 23 putatively known and two previously unidentified precursor ions. Moreover, through 16S rRNA-gene sequencing we were able to identify putatively novel bacterial species from the phyla Actinobacteria, Proteobacteria and also Firmicutes. Our findings suggest that sea cucumbers like H. leucospilota and S. vastus are promising sources for the isolation of novel bacterial species that produce compounds with potentially high biotechnological potential.en_US
dc.language.isoenen_US
dc.publisherMPDIen_US
dc.rightsAttribution-NonCommercial-ShareAlike 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.subjectanti-infective marine derived compoundsen_US
dc.subjectde-replicationen_US
dc.subjectmarine bacteriaen_US
dc.subjectmass spectrometryen_US
dc.subjectsea cucumberen_US
dc.titleBiotechnological Potential of Bacteria Isolated from the Sea Cucumber and from Lampung, Indonesia.en_US
dc.typeArticleen_US
dc.contributor.departmentHZI,Helmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7,38124 Braunschweig, Germany.en_US
dc.identifier.journalMarine Drugsen_US
refterms.dateFOA2019-11-21T09:44:30Z
dc.source.journaltitleMarine drugs


Files in this item

Thumbnail
Name:
Wibowo et al.pdf
Size:
1.374Mb
Format:
PDF
Description:
Open Access publication

This item appears in the following Collection(s)

Show simple item record

Attribution-NonCommercial-ShareAlike 4.0 International
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-ShareAlike 4.0 International