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dc.contributor.authorBohm, Katrin
dc.contributor.authorStrömpl, Julia
dc.contributor.authorKrumbholz, Andi
dc.contributor.authorZell, Roland
dc.contributor.authorKrause, Gérard
dc.contributor.authorSievers, Claudia
dc.date.accessioned2019-11-25T10:18:01Z
dc.date.available2019-11-25T10:18:01Z
dc.date.issued2019-11-06
dc.identifier.citationJ Clin Microbiol. 2019 Nov 6. pii: JCM.01029-19. doi: 10.1128/JCM.01029-19en_US
dc.identifier.issn1098-660X
dc.identifier.pmid31694975
dc.identifier.doi10.1128/JCM.01029-19
dc.identifier.urihttp://hdl.handle.net/10033/622027
dc.description.abstractHepatitis E, a liver disease caused by infection with the hepatitis E virus (HEV) is a worldwide emerging disease. The diagnosis is based on the detection of viral RNA and of HEV-specific immunoglobulins (Ig). For the latter, various assays are commercially available but still lack harmonization.In this study, a Luminex®-based multiplex serological assay was established, that measures the presence of total IgG, IgA and IgM antibodies, targeting a short peptide derived from the viral E2 protein. For the validation, 160 serum samples with a known HEV serostatus were used to determine the assay cut-off and accuracy. Thereby, HEV-IgG and -RNA positive sera were identified with a sensitivity of 100% and a specificity of 98% [CI95% 94-100%].Application of the assay by re-testing 514 sera previously characterised with different HEV-IgG or total antibody tests, revealed a high level of agreement between the assays (Cohens Kappa 0.58-0.99).The established method is highly sensitive, specific and can be easily implemented in a multiplex format to facilitate rapid differential diagnostics with a few microliters of sample input.en_US
dc.language.isoenen_US
dc.publisherASMen_US
dc.rightsAttribution-NonCommercial-ShareAlike 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.titleEstablishment of a highly sensitive assay for detection of Hepatitis E virus-specific immunoglobulins.en_US
dc.typeArticleen_US
dc.contributor.departmentHZI,Helmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7,38124 Braunschweig, Germany.en_US
dc.identifier.journalJournal of clinical microbiologyen_US
dc.source.journaltitleJournal of clinical microbiology


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