Establishment of a highly sensitive assay for detection of Hepatitis E virus-specific immunoglobulins.
dc.contributor.author | Bohm, Katrin | |
dc.contributor.author | Strömpl, Julia | |
dc.contributor.author | Krumbholz, Andi | |
dc.contributor.author | Zell, Roland | |
dc.contributor.author | Krause, Gérard | |
dc.contributor.author | Sievers, Claudia | |
dc.date.accessioned | 2019-11-25T10:18:01Z | |
dc.date.available | 2019-11-25T10:18:01Z | |
dc.date.issued | 2019-11-06 | |
dc.identifier.citation | J Clin Microbiol. 2019 Nov 6. pii: JCM.01029-19. doi: 10.1128/JCM.01029-19 | en_US |
dc.identifier.issn | 1098-660X | |
dc.identifier.pmid | 31694975 | |
dc.identifier.doi | 10.1128/JCM.01029-19 | |
dc.identifier.uri | http://hdl.handle.net/10033/622027 | |
dc.description.abstract | Hepatitis E, a liver disease caused by infection with the hepatitis E virus (HEV) is a worldwide emerging disease. The diagnosis is based on the detection of viral RNA and of HEV-specific immunoglobulins (Ig). For the latter, various assays are commercially available but still lack harmonization.In this study, a Luminex®-based multiplex serological assay was established, that measures the presence of total IgG, IgA and IgM antibodies, targeting a short peptide derived from the viral E2 protein. For the validation, 160 serum samples with a known HEV serostatus were used to determine the assay cut-off and accuracy. Thereby, HEV-IgG and -RNA positive sera were identified with a sensitivity of 100% and a specificity of 98% [CI95% 94-100%].Application of the assay by re-testing 514 sera previously characterised with different HEV-IgG or total antibody tests, revealed a high level of agreement between the assays (Cohens Kappa 0.58-0.99).The established method is highly sensitive, specific and can be easily implemented in a multiplex format to facilitate rapid differential diagnostics with a few microliters of sample input. | en_US |
dc.language.iso | en | en_US |
dc.publisher | ASM | en_US |
dc.rights | Attribution-NonCommercial-ShareAlike 4.0 International | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-sa/4.0/ | * |
dc.title | Establishment of a highly sensitive assay for detection of Hepatitis E virus-specific immunoglobulins. | en_US |
dc.type | Article | en_US |
dc.contributor.department | HZI,Helmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7,38124 Braunschweig, Germany. | en_US |
dc.identifier.journal | Journal of clinical microbiology | en_US |
dc.source.journaltitle | Journal of clinical microbiology |