No Evidence of Mosquito Involvement in the Transmission of Equine Hepacivirus (Flaviviridae) in an Epidemiological Survey of Austrian Horses
Cast your vote
You can rate an item by clicking the amount of stars they wish to award to this item.
When enough users have cast their vote on this item, the average rating will also be shown.
Your vote was cast
Thank you for your feedback
Thank you for your feedback
de Heus, Phebe
Cavalleri, Jessika M.V.
MetadataShow full item record
AbstractPrevalence studies have demonstrated a global distribution of equine hepacivirus (EqHV), a member of the family Flaviviridae. However, apart from a single case of vertical transmission, natural routes of EqHV transmission remain elusive. Many known flaviviruses are horizontally transmitted between hematophagous arthropods and vertebrate hosts. This study represents the first investigation of potential EqHV transmission by mosquitoes. More than 5000 mosquitoes were collected across Austria and analyzed for EqHV ribonucleic acid (RNA) by reverse transcription quantitative polymerase chain reaction (RT-qPCR). Concurrently, 386 serum samples from horses in eastern Austria were analyzed for EqHV-specific antibodies by luciferase immunoprecipitation system (LIPS) and for EqHV RNA by RT-qPCR. Additionally, liver-specific biochemistry parameters were compared between EqHV RNA-positive horses and EqHV RNA-negative horses. Phylogenetic analysis was conducted in comparison to previously published sequences from various origins. No EqHV RNA was detected in mosquito pools. Serum samples yielded an EqHV antibody prevalence of 45.9% (177/386) and RNA prevalence of 4.15% (16/386). EqHV RNA-positive horses had significantly higher glutamate dehydrogenase (GLDH) levels (p = 0.013) than control horses. Phylogenetic analysis showed high similarity between nucleotide sequences of EqHV in Austrian horses and EqHV circulating in other regions. Despite frequently detected evidence of EqHV infection in Austrian horses, no viral RNA was found in mosquitoes. It is therefore unlikely that mosquitoes are vectors of this flavivirus.
CitationViruses. 2019 Nov 1;11(11). pii: v11111014. doi: 10.3390/v11111014.
AffiliationTWINCORE, Zentrum für experimentelle und klinische Infektionsforschung GmbH,Feodor-Lynen Str. 7, 30625 Hannover, Germany.
The following license files are associated with this item:
- Creative Commons
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-ShareAlike 4.0 International
- First detection and frequent occurrence of Equine Hepacivirus in horses on the African continent.
- Authors: Badenhorst M, Tegtmeyer B, Todt D, Guthrie A, Feige K, Campe A, Steinmann E, Cavalleri JMV
- Issue date: 2018 Sep
- First identification and phylogenetic analysis of equine hepacivirus in Korea.
- Authors: Kim HS, Moon HW, Sung HW, Kwon HM
- Issue date: 2017 Apr
- Differential Infection Patterns and Recent Evolutionary Origins of Equine Hepaciviruses in Donkeys.
- Authors: Walter S, Rasche A, Moreira-Soto A, Pfaender S, Bletsa M, Corman VM, Aguilar-Setien A, García-Lacy F, Hans A, Todt D, Schuler G, Shnaiderman-Torban A, Steinman A, Roncoroni C, Veneziano V, Rusenova N, Sandev N, Rusenov A, Zapryanova D, García-Bocanegra I, Jores J, Carluccio A, Veronesi MC, Cavalleri JMV, Drosten C, Lemey P, Steinmann E, Drexler JF
- Issue date: 2017 Jan 1
- Further Evidence for in Utero Transmission of Equine Hepacivirus to Foals.
- Authors: Pronost S, Fortier C, Marcillaud-Pitel C, Tapprest J, Foursin M, Saunier B, Pitel PH, Paillot R, Hue ES
- Issue date: 2019 Dec 5
- First description of Theiler's disease-associated virus infection and epidemiological investigation of equine pegivirus and equine hepacivirus coinfection in Brazil.
- Authors: Figueiredo AS, de Moraes MVDS, Soares CC, Chalhoub FLL, de Filippis AMB, Dos Santos DRL, de Almeida FQ, Godoi TLOS, de Souza AM, Burdman TR, de Lemos ERS, Dos Reis JKP, Cruz OG, Pinto MA
- Issue date: 2019 Jul