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dc.contributor.authorBadenhorst, Marcha
dc.contributor.authorde Heus, Phebe
dc.contributor.authorAuer, Angelika
dc.contributor.authorRümenapf, Till
dc.contributor.authorTegtmeyer, Birthe
dc.contributor.authorKolodziejek, Jolanta
dc.contributor.authorNowotny, Norbert
dc.contributor.authorSteinmann, Eike
dc.contributor.authorCavalleri, Jessika M.V.
dc.creatorBadenhorst, M.
dc.date.accessioned2019-11-27T09:51:17Z
dc.date.available2019-11-27T09:51:17Z
dc.date.issued2019-11-01
dc.identifier.citationViruses. 2019 Nov 1;11(11). pii: v11111014. doi: 10.3390/v11111014.en_US
dc.identifier.pmid31683893
dc.identifier.doi10.3390/v11111014
dc.identifier.urihttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85074548047&origin=inward
dc.identifier.urihttp://hdl.handle.net/10033/622034
dc.description.abstractPrevalence studies have demonstrated a global distribution of equine hepacivirus (EqHV), a member of the family Flaviviridae. However, apart from a single case of vertical transmission, natural routes of EqHV transmission remain elusive. Many known flaviviruses are horizontally transmitted between hematophagous arthropods and vertebrate hosts. This study represents the first investigation of potential EqHV transmission by mosquitoes. More than 5000 mosquitoes were collected across Austria and analyzed for EqHV ribonucleic acid (RNA) by reverse transcription quantitative polymerase chain reaction (RT-qPCR). Concurrently, 386 serum samples from horses in eastern Austria were analyzed for EqHV-specific antibodies by luciferase immunoprecipitation system (LIPS) and for EqHV RNA by RT-qPCR. Additionally, liver-specific biochemistry parameters were compared between EqHV RNA-positive horses and EqHV RNA-negative horses. Phylogenetic analysis was conducted in comparison to previously published sequences from various origins. No EqHV RNA was detected in mosquito pools. Serum samples yielded an EqHV antibody prevalence of 45.9% (177/386) and RNA prevalence of 4.15% (16/386). EqHV RNA-positive horses had significantly higher glutamate dehydrogenase (GLDH) levels (p = 0.013) than control horses. Phylogenetic analysis showed high similarity between nucleotide sequences of EqHV in Austrian horses and EqHV circulating in other regions. Despite frequently detected evidence of EqHV infection in Austrian horses, no viral RNA was found in mosquitoes. It is therefore unlikely that mosquitoes are vectors of this flavivirus.en_US
dc.language.isoenen_US
dc.publisherMPDIen_US
dc.relation.ispartofViruses
dc.relation.ispartofseries11en_US
dc.rightsAttribution-NonCommercial-ShareAlike 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.subjectarbovirusen_US
dc.subjectflavivirusen_US
dc.subjecthematophagous arthropoden_US
dc.subjecthepacivirus Aen_US
dc.subjecthepatitisen_US
dc.subjectinsectsen_US
dc.subjectmosquito-borne virusen_US
dc.subjectvirus transmissionen_US
dc.titleNo Evidence of Mosquito Involvement in the Transmission of Equine Hepacivirus (Flaviviridae) in an Epidemiological Survey of Austrian Horsesen_US
dc.typeArticleen_US
dc.contributor.departmentTWINCORE, Zentrum für experimentelle und klinische Infektionsforschung GmbH,Feodor-Lynen Str. 7, 30625 Hannover, Germany.en_US
dc.identifier.journalVirusesen_US
dc.identifier.eid2-s2.0-85074548047
dc.identifier.scopusidSCOPUS_ID:85074548047
dc.relation.volume11


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