A Hypermorphic Allele Contributes to Impaired Thymic Deletion of Autoreactive Diabetogenic CD8 T Cells in NOD Mice.
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Authors
Presa, MaximilianoRacine, Jeremy J
Dwyer, Jennifer R
Lamont, Deanna J
Ratiu, Jeremy J
Sarsani, Vishal Kumar
Chen, Yi-Guang
Geurts, Aron
Schmitz, Ingo
Stearns, Timothy
Allocco, Jennifer
Chapman, Harold D
Serreze, David V
Issue Date
2018-10-01
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Show full item recordAbstract
In both NOD mice and humans, the development of type 1 diabetes (T1D) is dependent in part on autoreactive CD8+ T cells recognizing pancreatic β cell peptides presented by often quite common MHC class I variants. Studies in NOD mice previously revealed that the common H2-Kd and/or H2-Db class I molecules expressed by this strain aberrantly lose the ability to mediate the thymic deletion of pathogenic CD8+ T cell responses through interactions with T1D susceptibility genes outside the MHC. A gene(s) mapping to proximal chromosome 7 was previously shown to be an important contributor to the failure of the common class I molecules expressed by NOD mice to mediate the normal thymic negative selection of diabetogenic CD8+ T cells. Using an inducible model of thymic negative selection and mRNA transcript analyses, we initially identified an elevated Nfkbid expression variant as a likely NOD-proximal chromosome 7 region gene contributing to impaired thymic deletion of diabetogenic CD8+ T cells. CRISPR/Cas9-mediated genetic attenuation of Nfkbid expression in NOD mice resulted in improved negative selection of autoreactive diabetogenic AI4 and NY8.3 CD8+ T cells. These results indicated that allelic variants of Nfkbid contribute to the efficiency of intrathymic deletion of diabetogenic CD8+ T cells. However, although enhancing thymic deletion of pathogenic CD8+ T cells, ablating Nfkbid expression surprisingly accelerated T1D onset that was associated with numeric decreases in both regulatory T and B lymphocytes in NOD mice.Citation
J Immunol. 2018 Oct 1;201(7):1907-1917. doi: 10.4049/jimmunol.1800465. Epub 2018 Aug 20.Affiliation
HZI,Helmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7,38124 Braunschweig, Germany.Publisher
American Association of ImmunologistsJournal
Journal of ImmunologyPubMed ID
30127089PubMed Central ID
PMC6153649Additional Links
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6153649/Type
ArticleLanguage
enISSN
1550-6606ae974a485f413a2113503eed53cd6c53
10.4049/jimmunol.1800465
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