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dc.contributor.authorHercher, Thomas W
dc.contributor.authorKrausze, Joern
dc.contributor.authorHoffmeister, Sven
dc.contributor.authorZwerschke, Dagmar
dc.contributor.authorLindel, Thomas
dc.contributor.authorBlankenfeldt, Wulf
dc.contributor.authorMendel, Ralf R
dc.contributor.authorKruse, Tobias
dc.identifier.citationHercher, Thomas W., et al. "Insights into the Cnx1E catalyzed MPT-AMP hydrolysis." Bioscience Reports (2019): BSR20191806.Hercher, Thomas W., et al. "Insights into the Cnx1E catalyzed MPT-AMP hydrolysis." Bioscience Reports (2019): BSR20191806.en_US
dc.description.abstractMolybdenum insertases (Mo-insertases) catalyze the final step of molybdenum cofactor (Moco) biosynthesis, an evolutionary old and highly conserved multi-step pathway. In the first step of the pathway, GTP serves as substrate for the formation of cyclic pyranopterin monophosphate, which is subsequently converted into molybdopterin (MPT) in the second pathway step. In the following synthesis steps, MPT is adenylated yielding MPT-AMP that is subsequently used as substrate for enzyme catalyzed molybdate insertion. Molybdate insertion and MPT-AMP hydrolysis are catalyzed by the Mo-insertase E-domain. Earlier work reported a highly conserved aspartate residue to be essential for Mo-insertase functionality. In this work, we confirmed the mechanistic relevance of this residue for the Arabidopsis thaliana Mo-insertase Cnx1E. We found that the conservative substitution of Cnx1E residue Asp274 by Glu (D274E) leads to an arrest of MPT-AMP hydrolysis and hence to the accumulation of MPT-AMP. We further showed that the MPT-AMP accumulation goes in hand with the accumulation of molybdate. By crystallization and structure determination of the Cnx1E variant D274E, we identified the potential reason for the missing hydrolysis activity in the disorder of the region spanning amino acids 269 to 274. We reasoned that this is caused by the inability of a glutamate in position 274 to coordinate the octahedral Mg2+-water complex in the Cnx1E active site.en_US
dc.publisherPortland Pressen_US
dc.rightsAttribution-NonCommercial-ShareAlike 4.0 International*
dc.subjectAdenylated molybdopterinen_US
dc.subjectEnzyme catalyzed hydrolysisen_US
dc.subjectMolybdenum cofactoren_US
dc.subjectMolybdenum insertaseen_US
dc.titleInsights into the Cnx1E catalyzed MPT-AMP hydrolysis.en_US
dc.contributor.departmentHZI,Helmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7,38124 Braunschweig, Germany.en_US
dc.identifier.journalBioscience Reportsen_US
dc.source.journaltitleBioscience reports

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