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dc.contributor.authorWang, Chuan
dc.contributor.authorChao, Yanjie
dc.contributor.authorMatera, Gianluca
dc.contributor.authorGao, Qian
dc.contributor.authorVogel, Jörg
dc.date.accessioned2020-01-14T14:58:36Z
dc.date.available2020-01-14T14:58:36Z
dc.date.issued2019-12-21
dc.identifier.citationNucleic Acids Res. 2019 Dec 21. pii: 5682904. doi: 10.1093/nar/gkz1168.en_US
dc.identifier.issn1362-4962
dc.identifier.pmid31863581
dc.identifier.doi10.1093/nar/gkz1168
dc.identifier.urihttp://hdl.handle.net/10033/622083
dc.description.abstractSmall noncoding RNAs (sRNAs) from mRNA 3' UTRs seem to present a previously unrecognized layer of bacterial post-transcriptional control whereby mRNAs influence each other's expression, independently of transcriptional control. Studies in Escherichia coli and Salmonella enterica showed that such sRNAs are natural products of RNase E-mediated mRNA decay and associate with major RNA-binding proteins (RBPs) such as Hfq and ProQ. If so, there must be additional sRNAs from mRNAs that accumulate only under specific physiological conditions. We test this prediction by characterizing candidate NarS that represents the 3' UTR of nitrate transporter NarK whose gene is silent during standard aerobic growth. We find that NarS acts by Hfq-dependent base pairing to repress the synthesis of the nitrite transporter, NirC, resulting in mRNA cross-regulation of nitrate and nitrite transporter genes. Interestingly, the NarS-mediated repression selectively targets the nirC cistron of the long nirBDC-cysG operon, an observation that we rationalize as a mechanism to protect the bacterial cytoplasm from excessive nitrite toxicity during anaerobic respiration with abundant nitrate. Our successful functional assignment of a 3' UTR sRNA from a non-standard growth condition supports the notion that mRNA crossregulation is more pervasive than currently appreciated.en_US
dc.language.isoenen_US
dc.publisherOxford Academicen_US
dc.rightsAttribution-NonCommercial-ShareAlike 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.titleThe conserved 3' UTR-derived small RNA NarS mediates mRNA crossregulation during nitrate respiration.en_US
dc.typeArticleen_US
dc.contributor.departmentHIRI, Helmholtz-Institut für RNA-basierte Infektionsforschung, Josef-Shneider Strasse 2, 97080 Würzburg, Germany.en_US
dc.identifier.journalNucleic Acids Researchen_US
refterms.dateFOA2020-01-14T14:58:37Z
dc.source.journaltitleNucleic acids research


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Attribution-NonCommercial-ShareAlike 4.0 International
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