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dc.contributor.authorThöming, Janne G
dc.contributor.authorTomasch, Jürgen
dc.contributor.authorPreusse, Matthias
dc.contributor.authorKoska, Michal
dc.contributor.authorGrahl, Nora
dc.contributor.authorPohl, Sarah
dc.contributor.authorWillger, Sven D
dc.contributor.authorKaever, Volkhard
dc.contributor.authorMüsken, Mathias
dc.contributor.authorHäussler, Susanne
dc.identifier.citationNPJ Biofilms Microbiomes. 2020 Jan 10;6:2. doi: 10.1038/s41522-019-0113-6. eCollection 2020.en_US
dc.description.abstractStudying parallel evolution of similar traits in independent within-species lineages provides an opportunity to address evolutionary predictability of molecular changes underlying adaptation. In this study, we monitored biofilm forming capabilities, motility, and virulence phenotypes of a plethora of phylogenetically diverse clinical isolates of the opportunistic pathogen Pseudomonas aeruginosa. We also recorded biofilm-specific and planktonic transcriptional responses. We found that P. aeruginosa isolates could be stratified based on the production of distinct organismal traits. Three major biofilm phenotypes, which shared motility and virulence phenotypes, were produced repeatedly in several isolates, indicating that the phenotypes evolved via parallel or convergent evolution. Of note, while we found a restricted general response to the biofilm environment, the individual groups of biofilm phenotypes reproduced biofilm transcriptional profiles that included the expression of well-known biofilm features, such as surface adhesive structures and extracellular matrix components. Our results provide insights into distinct ways to make a biofilm and indicate that genetic adaptations can modulate multiple pathways for biofilm development that are followed by several independent clinical isolates. Uncovering core regulatory pathways that drive biofilm-associated growth and tolerance towards environmental stressors promises to give clues to host and environmental interactions and could provide useful targets for new clinical interventions.en_US
dc.publisherNature publishing groupen_US
dc.rightsAttribution-NonCommercial-ShareAlike 4.0 International*
dc.subjectNext-generation sequencingen_US
dc.titleParallel evolutionary paths to produce more than one biofilm phenotype.en_US
dc.contributor.departmentHZI,Helmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7,38124 Braunschweig, Germany.en_US
dc.identifier.journalNPJ Biofilms and Microbiomes.en_US
dc.source.journaltitleNPJ biofilms and microbiomes

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