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dc.contributor.authorJaber, Lina
dc.contributor.authorAmro, Mahmod
dc.contributor.authorTair, Hadeel Abu
dc.contributor.authorBahader, Shereen A
dc.contributor.authorAlalam, Hanna
dc.contributor.authorButmeh, Suha
dc.contributor.authorHilal, Dalia Abu
dc.contributor.authorBrettar, Ingrid
dc.contributor.authorHöfle, Manfred G
dc.contributor.authorBitar, Dina M
dc.date.accessioned2020-01-30T10:51:02Z
dc.date.available2020-01-30T10:51:02Z
dc.date.issued2018-01-01
dc.identifier.citationEpidemiol Infect. 2018 Dec;146(16):2116-2121. doi: 10.1017/S0950268818002340 Epub 2018 Aug 30.en_US
dc.identifier.issn1469-4409
dc.identifier.pmid30157982
dc.identifier.doi10.1017/S0950268818002340
dc.identifier.urihttp://hdl.handle.net/10033/622109
dc.description.abstractLegionella pneumophila genotyping is important for epidemiological investigation of nosocomial and community-acquired outbreaks of legionellosis. The prevalence of legionellosis in pneumonia patients in the West Bank was monitored for the first time, and the sequence types (STs) from respiratory samples were compared with STs of environmental samples from different wards of the hospital. Sputum (n = 121) and bronchoalveolar lavage (BAL) (n = 74) specimens were cultured for L. pneumophila; genomic DNA was tested by 16S rRNA polymerase chain reaction (PCR) amplification. Nested PCR sequence-based typing (NPSBT) was implemented on DNA of the respiratory and environmental PCR-positive samples. Only one respiratory specimen was positive for L. pneumophila by culture. BAL gave a higher percentage of L. pneumophila-positive samples, 35% (26/74) than sputum, 15% (18/121) by PCR. NPSBT revealed the following STs: ST 1 (29%, 7/24), ST 461 (21%, 5/24), ST 1037 (4%, 1/24) from respiratory samples, STs from environmental samples: ST 1 (28.5%, 4/14), ST 187 (21.4%, 3/14) and ST 2070, ST 461, ST 1482 (7.1%, 1/14) each. This study emphasises the advantage of PCR over culture for the detection of L. pneumophila in countries where antibiotics are indiscriminately used prior to hospital admission. ST 1 was the predominant ST in both respiratory and environmental samples.en_US
dc.language.isoenen_US
dc.publisherCambridge University Pressen_US
dc.relation.urlhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6453007/en_US
dc.rightsAttribution-NonCommercial-ShareAlike 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.subjectEast Jerusalemen_US
dc.subjectWest Banken_US
dc.subjectlegionellosisen_US
dc.subjectsequence-based typingen_US
dc.titleComparison of in situ sequence type analysis of Legionella pneumophila in respiratory tract secretions and environmental samples of a hospital in East Jerusalem.en_US
dc.typeArticleen_US
dc.contributor.departmentHZI,Helmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7,38124 Braunschweig, Germany.en_US
dc.identifier.journalEpidemiology and Infectionen_US
refterms.dateFOA2020-01-30T10:51:02Z
dc.source.journaltitleEpidemiology and infection


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