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dc.contributor.authorRohmeier, L
dc.contributor.authorPetzl, W
dc.contributor.authorKoy, M
dc.contributor.authorEickhoff, T
dc.contributor.authorHülsebusch, A
dc.contributor.authorJander, S
dc.contributor.authorMacias, L
dc.contributor.authorHeimes, A
dc.contributor.authorEngelmann, S
dc.contributor.authorHoedemaker, M
dc.contributor.authorSeyfert, H M
dc.contributor.authorKühn, C
dc.contributor.authorSchuberth, H J
dc.contributor.authorZerbe, H
dc.contributor.authorMeyerholz, M M
dc.date.accessioned2020-02-10T09:10:13Z
dc.date.available2020-02-10T09:10:13Z
dc.date.issued2020-01-31
dc.identifier.citationBMC Vet Res. 2020 Jan 31;16(1):33. doi: 10.1186/s12917-020-2251-8.en_US
dc.identifier.issn1746-6148
dc.identifier.pmid32005239
dc.identifier.doi10.1186/s12917-020-2251-8
dc.identifier.urihttp://hdl.handle.net/10033/622123
dc.description.abstractBACKGROUND: In dairy herds, mastitis causes detrimental economic losses. Genetic selection offers a sustainable tool to select animals with reduced susceptibility towards postpartum diseases. Studying underlying mechanisms is important to assess the physiological processes that cause differences between selected haplotypes. Therefore, the objective of this study was to establish an in vivo infection model to study the impact of selecting for alternative paternal haplotypes in a particular genomic region on cattle chromosome 18 for mastitis susceptibility under defined conditions in uniparous dairy cows. RESULTS: At the start of pathogen challenge, no significant differences between the favorable (Q) and unfavorable (q) haplotypes were detected. Intramammary infection (IMI) with Staphylococcus aureus 1027 (S. aureus, n = 24, 96 h) or Escherichia coli 1303 (E. coli, n = 12, 24 h) was successfully induced in all uniparous cows. This finding was confirmed by clinical signs of mastitis and repeated recovery of the respective pathogen from milk samples of challenged quarters in each animal. After S. aureus challenge, Q-uniparous cows showed lower somatic cell counts 24 h and 36 h after challenge (P < 0.05), lower bacterial shedding in milk 12 h after challenge (P < 0.01) and a minor decrease in total milk yield 12 h and 24 h after challenge (P < 0.01) compared to q-uniparous cows. CONCLUSION: An in vivo infection model to study the impact of genetic selection for mastitis susceptibility under defined conditions in uniparous dairy cows was successfully established and revealed significant differences between the two genetically selected haplotype groups. This result might explain their differences in susceptibility towards IMI. These clinical findings form the basis for further in-depth molecular analysis to clarify the underlying genetic mechanisms for mastitis resistance.en_US
dc.language.isoenen_US
dc.publisherBioMed Central (BMC)en_US
dc.rightsAttribution-NonCommercial-ShareAlike 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.subjectBTA18en_US
dc.subjectEscherichia colien_US
dc.subjectGenetic selectionen_US
dc.subjectHaplotypeen_US
dc.subjectIntramammary infection modelen_US
dc.subjectMastitisen_US
dc.subjectSomatic cell counten_US
dc.subjectStaphylococcus aureusen_US
dc.titleIn vivo model to study the impact of genetic variation on clinical outcome of mastitis in uniparous dairy cows.en_US
dc.typeArticleen_US
dc.contributor.departmentHZI,Helmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7,38124 Braunschweig, Germany.en_US
dc.identifier.journalBMC Veterynary Researchen_US
refterms.dateFOA2020-02-10T09:10:13Z
dc.source.journaltitleBMC veterinary research


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