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dc.contributor.authorLapinaite, Audrone
dc.contributor.authorCarlomagno, Teresa
dc.contributor.authorGabel, Frank
dc.date.accessioned2020-02-11T14:17:33Z
dc.date.available2020-02-11T14:17:33Z
dc.date.issued2020-01-01
dc.identifier.citationMethods Mol Biol. 2020;2113:165-188. doi: 10.1007/978-1-0716-0278-2_13.en_US
dc.identifier.issn1940-6029
dc.identifier.pmid32006315
dc.identifier.doi10.1007/978-1-0716-0278-2_13
dc.identifier.urihttp://hdl.handle.net/10033/622126
dc.description.abstractSmall-angle neutron scattering (SANS) provides structural information on biomacromolecules and their complexes in dilute solutions at the nanometer length scale. The overall dimensions, shapes, and interactions can be probed and compared to information obtained by complementary structural biology techniques such as crystallography, NMR, and EM. SANS, in combination with solvent H2O/D2O exchange and/or deuteration, is particularly well suited to probe the internal structure of RNA-protein (RNP) complexes since neutrons are more sensitive than X-rays to the difference in scattering length densities of proteins and RNA, with respect to an aqueous solvent. In this book chapter we provide a practical guide on how to carry out SANS experiments on RNP complexes, as well as possibilities of data analysis and interpretation.en_US
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.rightsAttribution-NonCommercial-ShareAlike 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.subjectContrast variationen_US
dc.subjectDeuterium labelingen_US
dc.subjectMacromolecular complexen_US
dc.subjectProteinen_US
dc.subjectRNAen_US
dc.subjectRNPen_US
dc.subjectSmall-angle neutron scattering (SANS)en_US
dc.subjectStructural biologyen_US
dc.titleSmall-Angle Neutron Scattering of RNA-Protein Complexes.en_US
dc.typeArticleen_US
dc.contributor.departmentHZI,Helmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7,38124 Braunschweig, Germany.en_US
dc.identifier.journalMethods in molecular biologyen_US
dc.source.journaltitleMethods in molecular biology (Clifton, N.J.)


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