Reproducible Colonization of Germ-Free Mice With the Oligo-Mouse-Microbiota in Different Animal Facilities.
Cast your vote
You can rate an item by clicking the amount of stars they wish to award to this item.
When enough users have cast their vote on this item, the average rating will also be shown.
Your vote was cast
Thank you for your feedback
Thank you for your feedback
Münch, Philipp C
Slack, Emma Caroline
Frick, Julia S
MetadataShow full item record
AbstractThe Oligo-Mouse-Microbiota (OMM12) is a recently developed synthetic bacterial community for functional microbiome research in mouse models (Brugiroux et al., 2016). To date, the OMM12 model has been established in several germ-free mouse facilities world-wide and is employed to address a growing variety of research questions related to infection biology, mucosal immunology, microbial ecology and host-microbiome metabolic cross-talk. The OMM12 consists of 12 sequenced and publically available strains isolated from mice, representing five bacterial phyla that are naturally abundant in the murine gastrointestinal tract (Lagkouvardos et al., 2016). Under germ-free conditions, the OMM12 colonizes mice stably over multiple generations. Here, we investigated whether stably colonized OMM12 mouse lines could be reproducibly established in different animal facilities. Germ-free C57Bl/6J mice were inoculated with a frozen mixture of the OMM12 strains. Within 2 weeks after application, the OMM12 community reached the same stable composition in all facilities, as determined by fecal microbiome analysis. We show that a second application of the OMM12 strains after 72 h leads to a more stable community composition than a single application. The availability of such protocols for reliable de novo generation of gnotobiotic rodents will certainly contribute to increasing experimental reproducibility in biomedical research.
CitationFront Microbiol. 2020 Jan 10;10:2999. doi: 10.3389/fmicb.2019.02999. eCollection 2019.
AffiliationBRICS, Braunschweiger Zentrum für Systembiologie, Rebenring 56,38106 Braunschweig, Germany.
JournalFrontiers in Microbiology
The following license files are associated with this item:
- Creative Commons
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-ShareAlike 4.0 International
- Composition of the Intestinal Microbiota Determines the Outcome of Virus-Triggered Colitis in Mice.
- Authors: Bolsega S, Basic M, Smoczek A, Buettner M, Eberl C, Ahrens D, Odum KA, Stecher B, Bleich A
- Issue date: 2019
- Genome-guided design of a defined mouse microbiota that confers colonization resistance against Salmonella enterica serovar Typhimurium.
- Authors: Brugiroux S, Beutler M, Pfann C, Garzetti D, Ruscheweyh HJ, Ring D, Diehl M, Herp S, Lötscher Y, Hussain S, Bunk B, Pukall R, Huson DH, Münch PC, McHardy AC, McCoy KD, Macpherson AJ, Loy A, Clavel T, Berry D, Stecher B
- Issue date: 2016 Nov 21
- The effect of diet on the human gut microbiome: a metagenomic analysis in humanized gnotobiotic mice.
- Authors: Turnbaugh PJ, Ridaura VK, Faith JJ, Rey FE, Knight R, Gordon JI
- Issue date: 2009 Nov 11
- Standardization in host-microbiota interaction studies: challenges, gnotobiology as a tool, and perspective.
- Authors: Mooser C, Gomez de Agüero M, Ganal-Vonarburg SC
- Issue date: 2018 Aug
- Functional Intestinal Bile Acid 7α-Dehydroxylation by <i>Clostridium scindens</i> Associated with Protection from <i>Clostridium difficile</i> Infection in a Gnotobiotic Mouse Model.
- Authors: Studer N, Desharnais L, Beutler M, Brugiroux S, Terrazos MA, Menin L, Schürch CM, McCoy KD, Kuehne SA, Minton NP, Stecher B, Bernier-Latmani R, Hapfelmeier S
- Issue date: 2016