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dc.contributor.authorEberl, Claudia
dc.contributor.authorRing, Diana
dc.contributor.authorMünch, Philipp C
dc.contributor.authorBeutler, Markus
dc.contributor.authorBasic, Marijana
dc.contributor.authorSlack, Emma Caroline
dc.contributor.authorSchwarzer, Martin
dc.contributor.authorSrutkova, Dagmar
dc.contributor.authorLange, Anna
dc.contributor.authorFrick, Julia S
dc.contributor.authorBleich, André
dc.contributor.authorStecher, Bärbel
dc.date.accessioned2020-02-17T09:47:34Z
dc.date.available2020-02-17T09:47:34Z
dc.date.issued2019-01-01
dc.identifier.citationFront Microbiol. 2020 Jan 10;10:2999. doi: 10.3389/fmicb.2019.02999. eCollection 2019.en_US
dc.identifier.issn1664-302X
dc.identifier.pmid31998276
dc.identifier.doi10.3389/fmicb.2019.02999
dc.identifier.urihttp://hdl.handle.net/10033/622139
dc.description.abstractThe Oligo-Mouse-Microbiota (OMM12) is a recently developed synthetic bacterial community for functional microbiome research in mouse models (Brugiroux et al., 2016). To date, the OMM12 model has been established in several germ-free mouse facilities world-wide and is employed to address a growing variety of research questions related to infection biology, mucosal immunology, microbial ecology and host-microbiome metabolic cross-talk. The OMM12 consists of 12 sequenced and publically available strains isolated from mice, representing five bacterial phyla that are naturally abundant in the murine gastrointestinal tract (Lagkouvardos et al., 2016). Under germ-free conditions, the OMM12 colonizes mice stably over multiple generations. Here, we investigated whether stably colonized OMM12 mouse lines could be reproducibly established in different animal facilities. Germ-free C57Bl/6J mice were inoculated with a frozen mixture of the OMM12 strains. Within 2 weeks after application, the OMM12 community reached the same stable composition in all facilities, as determined by fecal microbiome analysis. We show that a second application of the OMM12 strains after 72 h leads to a more stable community composition than a single application. The availability of such protocols for reliable de novo generation of gnotobiotic rodents will certainly contribute to increasing experimental reproducibility in biomedical research.en_US
dc.language.isoenen_US
dc.publisherFrontiersen_US
dc.rightsAttribution-NonCommercial-ShareAlike 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.subject3Ren_US
dc.subjectOligo-MM12en_US
dc.subjectdefined bacterial consortiaen_US
dc.subjectgnotobiologyen_US
dc.subjectisobiotic miceen_US
dc.subjectminimal microbiomeen_US
dc.subjectsDMDMm2en_US
dc.subjectsyncomen_US
dc.titleReproducible Colonization of Germ-Free Mice With the Oligo-Mouse-Microbiota in Different Animal Facilities.en_US
dc.typeArticleen_US
dc.contributor.departmentBRICS, Braunschweiger Zentrum für Systembiologie, Rebenring 56,38106 Braunschweig, Germany.en_US
dc.identifier.journalFrontiers in Microbiologyen_US
refterms.dateFOA2020-02-17T09:47:34Z
dc.source.journaltitleFrontiers in microbiology


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