Identification of antibiotics that diminish disease in a murine model of enterohemorrhagic infection.
Cast your vote
You can rate an item by clicking the amount of stars they wish to award to this item.
When enough users have cast their vote on this item, the average rating will also be shown.
Your vote was cast
Thank you for your feedback
Thank you for your feedback
Pils, Marina C
MetadataShow full item record
AbstractInfections with enterohemorrhagic Escherichia coli (EHEC) cause disease ranging from mild diarrhea to hemolytic uremic syndrome (HUS) and are the most common cause of renal failure in children in high income countries. The severity of the disease derives from the release of Shiga toxins (Stx). The use of antibiotics to treat EHEC infections is generally avoided as it can result in increased stx expression. Here, we systematically tested different classes of antibiotics and found that their influence on stx expression and release varies significantly. We assessed a selection of these antibiotics in vivo using the Citrobacter rodentium φstx2dact mouse model and show that stx2d-inducing antibiotics resulted in weight loss and kidney damage despite clearing the infection. However, several non-Stx-inducing antibiotics cleared bacterial infection without causing Stx-mediated pathology. Our results suggest that these antibiotics could be useful in the treatment of EHEC-infected human patients and decrease the risk of HUS development.
CitationAntimicrob Agents Chemother. 2020 Feb 3. pii: AAC.02159-19. doi: 10.1128/AAC.02159-19.
AffiliationHZI,Helmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7,38124 Braunschweig, Germany.
PublisherAmerican society for microbiology
The following license files are associated with this item:
- Creative Commons
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-ShareAlike 4.0 International
- Shiga toxin in enterohemorrhagic E.coli: regulation and novel anti-virulence strategies.
- Authors: Pacheco AR, Sperandio V
- Issue date: 2012
- Shiga toxin-mediated hemolytic uremic syndrome: time to change the diagnostic paradigm?
- Authors: Bielaszewska M, Köck R, Friedrich AW, von Eiff C, Zimmerhackl LB, Karch H, Mellmann A
- Issue date: 2007 Oct 10
- A novel murine infection model for Shiga toxin-producing Escherichia coli.
- Authors: Mallick EM, McBee ME, Vanguri VK, Melton-Celsa AR, Schlieper K, Karalius BJ, O'Brien AD, Butterton JR, Leong JM, Schauer DB
- Issue date: 2012 Nov
- Synchronous Disease Kinetics in a Murine Model for Enterohemorrhagic <i>E. coli</i> Infection Using Food-Borne Inoculation.
- Authors: Flowers LJ, Bou Ghanem EN, Leong JM
- Issue date: 2016
- Promoter sequence of Shiga toxin 2 (Stx2) is recognized in vivo, leading to production of biologically active Stx2.
- Authors: Bentancor LV, Mejías MP, Pinto A, Bilen MF, Meiss R, Rodriguez-Galán MC, Baez N, Pedrotti LP, Goldstein J, Ghiringhelli PD, Palermo MS
- Issue date: 2013 Oct 1